Du Xihao, Jiang Shuo, Bo Liang, Liu Jie, Zeng Xuejiao, Xie Yuquan, He Qing, Ye Xingwang, Song Weiming, Zhao Jinzhuo
Department of Environmental Health, School of Public Health and the Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai, 200032, China.
Department of Cardiology, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 200092, China.
Chemosphere. 2017 Apr;173:14-21. doi: 10.1016/j.chemosphere.2017.01.042. Epub 2017 Jan 8.
This study aims to observe whether the combined treatment with vitamin E (vit E) and omega-3 polyunsaturated fatty acids (Ω-3 FA) could prevent the fine particulate matter (PM)-induced cardiovascular injury through alleviating inflammation and oxidative stress. At the same time, the appropriate combination dosage of vit E and Ω-3 FA was explored to find an optimized protective dose to protect the injury induced by PM.
The SD rats were pretreated with different concentration of vit E and Ω-3 FA separately or jointly. Then the rats were exposed to ambient PM by intratracheal instillation for three times. The expression of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) in serum and supernatant of cardiac tissue were detected by ELISA kits. The levels of malondialdehyde (MDA), superoxide Dismutase (SOD) and glutathione-peroxidase (GSH-Px) in myocardium and the level of MDA in serum were measured. Meanwhile, the cardiac injury was evaluated by histopathological examination.
Compared with the severe injury of rats in PM exposure group, the rats in vit E or Ω-3 FA-pretreated groups had a slighter injury in heart. Meanwhile, pretreatment with vit E or Ω-3 FA induced a significantly alleviation of the inflammatory cytokines (TNF-α, IL-1β, IL-6) and the elevation of the anti-oxidative activity especially in the rats pretreated with combined vit E and Ω-3 FA. In addition, the combined protecting effects of vit E and Ω-3 FA showed a dose-dependent manner.
Supplementation with vit E and Ω-3 FA could protect the PM-induced injury, and the combination of vit E and Ω-3 FA might produce more effective effects than the separate nutrient did.
本研究旨在观察维生素E（vit E）与ω-3多不饱和脂肪酸（Ω-3 FA）联合治疗是否能通过减轻炎症和氧化应激来预防细颗粒物（PM）诱导的心血管损伤。同时，探索vit E与Ω-3 FA的合适联合剂量，以找到优化的保护剂量来保护PM诱导的损伤。
将SD大鼠分别或联合用不同浓度的vit E和Ω-3 FA进行预处理。然后通过气管内滴注使大鼠暴露于环境PM中三次。用ELISA试剂盒检测血清和心脏组织上清液中肿瘤坏死因子α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）的表达。测量心肌中丙二醛（MDA）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的水平以及血清中MDA的水平。同时，通过组织病理学检查评估心脏损伤。
与PM暴露组大鼠的严重损伤相比，vit E或Ω-3 FA预处理组的大鼠心脏损伤较轻。同时，vit E或Ω-3 FA预处理可显著减轻炎性细胞因子（TNF-α、IL-1β、IL-6），并提高抗氧化活性，尤其是在联合使用vit E和Ω-3 FA预处理的大鼠中。此外，vit E和Ω-3 FA的联合保护作用呈剂量依赖性。
补充vit E和Ω-3 FA可保护PM诱导的损伤，且vit E和Ω-3 FA联合使用可能比单独使用营养素产生更有效的效果。
