Environmental Biotechnology Division, Environmental Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow 226001, Uttar Pradesh, India; Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Lucknow, India.
Environmental Biotechnology Division, Environmental Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow 226001, Uttar Pradesh, India.
Bioresour Technol. 2019 Aug;285:121314. doi: 10.1016/j.biortech.2019.121314. Epub 2019 Apr 2.
Biosurfactants from the yeast strains Candida albicans SC5314 and Candida glabrata CBS138 were isolated and characterized. Surface tension of the cell-free broth was reduced from 72 N/m to 42 N/m and 55 N/m respectively. The biosurfactants showed emulsifying ability as the indices against castor oil were determined to be 51% and 53% for C. albicans and C. glabrata respectively and were found stable between pH 2 and 10, temperature 4-120 °C and salt concentration 2-14%. The partially purified surfactants were identified as sophorolipid using Fourier transform infrared spectroscopy. Isolated sophorolipid showed antibacterial properties against pathogenic bacteria and generated reactive oxygen species in Bacillus subtilis and Escherichia coli. Flow cytometric analysis revealed that 60 mg/L of C. glabrata biosurfactant killed 65.8% B. subtilis and 4% E. coli. The data here obtained indicates applications of biosurfactant focusing mainly as antimicrobial and therapeutic perspectives.
从酵母菌株白色念珠菌 SC5314 和光滑念珠菌 CBS138 中分离和鉴定了生物表面活性剂。无细胞发酵液的表面张力分别降低到 72N/m 和 55N/m。生物表面活性剂具有乳化能力，其对蓖麻油的指数分别为 51%和 53%，在 pH 值为 2 到 10、温度为 4 到 120°C 和盐浓度为 2 到 14%的条件下稳定。部分纯化的表面活性剂通过傅里叶变换红外光谱鉴定为槐糖脂。分离的槐糖脂对病原菌具有抗菌活性，并在枯草芽孢杆菌和大肠杆菌中产生活性氧。流式细胞术分析表明，60mg/L 的 C. glabrata 生物表面活性剂可杀死 65.8%的枯草芽孢杆菌和 4%的大肠杆菌。此处获得的数据表明，生物表面活性剂的应用主要集中在抗菌和治疗方面。
