绵羊红细胞特异性B细胞前体。在特定辅助检测中频率的估计。
B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay.
作者信息
Schreier M H
出版信息
J Exp Med. 1978 Dec 1;148(6):1612-9. doi: 10.1084/jem.148.6.1612.
Abstract
A sensitive, specific, and reproducible in vitro helper assay is described which is suited to limiting dilution analysis of murine B cells. 1 in about 3,000 syngeneic splenic B cells can be induced to form plaque-forming cells (PFC) to sheep erythrocytes in this system. The induction of PFC is absolutely dependent on antigen and specific helper T cells.
摘要
本文描述了一种灵敏、特异且可重复的体外辅助检测方法，该方法适用于对小鼠B细胞进行有限稀释分析。在该系统中，约3000个同基因脾B细胞中的1个可被诱导形成针对绵羊红细胞的空斑形成细胞（PFC）。PFC的诱导绝对依赖于抗原和特异性辅助性T细胞。
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B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay.绵羊红细胞特异性B细胞前体。在特定辅助检测中频率的估计。
J Exp Med. 1978 Dec 1;148(6):1612-9. doi: 10.1084/jem.148.6.1612.
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Restricted helper function of F1 hybrid T cells positively selected to heterologous erythrocytes in irradiated parental strain mice. I. Failure to collaborate with B cells of the opposite parental strain not associated with active suppression.在经辐照的亲代品系小鼠中对异源红细胞呈阳性选择的F1杂种T细胞的受限辅助功能。I. 未能与不伴有主动抑制的相反亲代品系的B细胞协作。
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引用本文的文献
1
T-cell-dependent B-cell stimulation is H-2 restricted and antigen dependent only at the resting B-cell level.T细胞依赖性B细胞刺激是H-2限制性的，并且仅在静止B细胞水平上依赖抗原。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1612-6. doi: 10.1073/pnas.77.3.1612.
2
Antigen-specific T-helper cells stimulate H-2-compatible and H-2-incompatible B-cell blasts polyclonally.抗原特异性辅助性T细胞可多克隆地刺激H-2相容和H-2不相容的B细胞母细胞。
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Control of B cell differentiation by T cells.T细胞对B细胞分化的调控。
Surv Immunol Res. 1983;2(3):216-9. doi: 10.1007/BF02918415.
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Quantitative studies on T cell diversity. III. Limiting dilution analysis of precursor cells for T helper cells reactive to xenogeneic erythrocytes.T细胞多样性的定量研究。III. 对异种红细胞反应性T辅助细胞前体细胞的有限稀释分析。
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Proc Natl Acad Sci U S A. 1989 Aug;86(15):5728-32. doi: 10.1073/pnas.86.15.5728.
本文引用的文献
1
The thymus and the precursors of antigen reactive cells.胸腺与抗原反应性细胞的前体。
Nature. 1967 Nov 18;216(5116):659-63. doi: 10.1038/216659a0.
2
Immunization of dissociated spleen cell cultures from normal mice.对来自正常小鼠的脾细胞解离培养物进行免疫接种。
J Exp Med. 1967 Sep 1;126(3):423-42. doi: 10.1084/jem.126.3.423.
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Induction of a hemolysin response in vitro. Interaction of cells of bone marrow origin and thymic origin.体外溶血素反应的诱导。骨髓来源细胞与胸腺来源细胞的相互作用。
J Exp Med. 1970 Dec 1;132(6):1267-78. doi: 10.1084/jem.132.6.1267.
4
Differentiation of lymphocytes in mouse bone marrow. I. Quantitative radioautographic studies of antiglobulin binding by lymphocytes in bone marrow and lymphoid tissues.小鼠骨髓中淋巴细胞的分化。I. 骨髓和淋巴组织中淋巴细胞抗球蛋白结合的定量放射自显影研究。
Cell Immunol. 1974 Jul;13(1):117-31. doi: 10.1016/0008-8749(74)90232-9.
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Precursor cells specific to sheep red cells in nude mice. Estimation of frequency in the microculture system.裸鼠中绵羊红细胞特异性前体细胞。微培养系统中频率的估计。
Eur J Immunol. 1973 Jul;3(7):392-7. doi: 10.1002/eji.1830030704.
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Radioresistance of cooperative function of carrier-specific lymphocytes in antihapten antibody responses.抗半抗原抗体应答中载体特异性淋巴细胞协同功能的放射抗性
Science. 1970 Oct 23;170(3956):462-4. doi: 10.1126/science.170.3956.462.
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Partial replacement of serum by selenite, transferrin, albumin and lecithin in haemopoietic cell cultures.造血细胞培养中用亚硒酸盐、转铁蛋白、白蛋白和卵磷脂部分替代血清。
Nature. 1976 Oct 14;263(5578):594-5. doi: 10.1038/263594a0.
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Frequencies of mitogen-reactive B cells in the mouse. II. Frequencies of B cells producing antibodies which lyse sheep or horse erythrocytes, and trinitrophenylated or nitroiodophenylated sheep erythrocytes.小鼠中有丝分裂原反应性B细胞的频率。II. 产生能裂解绵羊或马红细胞以及三硝基苯基化或硝基碘苯基化绵羊红细胞的抗体的B细胞频率。
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