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抗原特异性辅助性T细胞可多克隆地刺激H-2相容和H-2不相容的B细胞母细胞。

Antigen-specific T-helper cells stimulate H-2-compatible and H-2-incompatible B-cell blasts polyclonally.

作者信息

Schreier M H, Andersson J, Lernhardt W, Melchers F

出版信息

J Exp Med. 1980 Jan 1;151(1):194-203. doi: 10.1084/jem.151.1.194.

DOI:10.1084/jem.151.1.194
PMID:6965306
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2185768/
Abstract

Lipopolysaccharide (LPS)-activated B-cell blasts from C57BL/6J nu/nu spleen cells develop into IgM-secreting clones after stimulation by antigen-specific T-helper cells of C57BL/6J origin. Although induction of help is antigen-dependent, help itself acts polyclonally. 1 of 1--3 B-cell blasts is restimulated in a homologous fashion by LPS, or in a heterologous fashion by sheep erythrocyte (SRC)- or horse erythrocyte (HRC)-activated T-helper cells. The repertoire of activated B-cell blasts reflects the polyclonal nature of activation: approximately 1 in 1,000--3,000 restimulated B-cell blasts is specific for SRC, 1 in 300--1,000 is specific for HRC, and 1 in 100--300 specific for trinitrophenylated SRC (TNP30-SRC). B-cell blasts that are either H-2 compatible or H-2 incompatible with the antigen-activated T-cell help are stimulated polyclonally in similar high frequencies. Thus, neither antigen nor H-2 compatibility are required to stimulate a B-cell blast into the next cell cycle.

摘要

来自C57BL/6J裸鼠脾脏细胞的脂多糖（LPS）激活的B细胞母细胞，在受到C57BL/6J来源的抗原特异性辅助性T细胞刺激后，会发育成分泌IgM的克隆。虽然辅助作用的诱导依赖于抗原，但辅助作用本身具有多克隆性。1/1至3个B细胞母细胞以同源方式被LPS再次刺激，或以异源方式被绵羊红细胞（SRC）或马红细胞（HRC）激活的辅助性T细胞再次刺激。被激活的B细胞母细胞的库反映了激活的多克隆性质：在1000至3000个再次刺激的B细胞母细胞中，约1个对SRC特异，300至1000个中有1个对HRC特异，100至300个中有1个对三硝基苯化SRC（TNP30-SRC）特异。与抗原激活的T细胞辅助作用H-2相容或H-2不相容的B细胞母细胞，以相似的高频率被多克隆刺激。因此，无论是抗原还是H-2相容性，都不是刺激B细胞母细胞进入下一个细胞周期所必需的。

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本文引用的文献

1
The carrier effect in the secondary response to hapten-protein conjugates. II. Cellular cooperation.对半抗原-蛋白质偶联物二次应答中的载体效应。II. 细胞协作。
Eur J Immunol. 1971 Jan;1(1):18-27. doi: 10.1002/eji.1830010104.
2
A requirement for two cell types for antibody formation in vitro.体外抗体形成需要两种细胞类型。
Science. 1967 Dec 22;158(3808):1573-5. doi: 10.1126/science.158.3808.1573.
3
Separation of cells by velocity sedimentation.通过速度沉降法分离细胞。
J Cell Physiol. 1969 Jun;73(3):191-201. doi: 10.1002/jcp.1040730305.
4
Induction of a hemolysin response in vitro. Interaction of cells of bone marrow origin and thymic origin.体外溶血素反应的诱导。骨髓来源细胞与胸腺来源细胞的相互作用。
J Exp Med. 1970 Dec 1;132(6):1267-78. doi: 10.1084/jem.132.6.1267.
5
Thymus and antigen-reactive cells.胸腺与抗原反应性细胞。
Transplant Rev. 1969;1:3-42. doi: 10.1111/j.1600-065x.1969.tb00135.x.
6
Immunoglobulins as cell receptors.作为细胞受体的免疫球蛋白。
Ann N Y Acad Sci. 1971 Dec 31;190:420-31. doi: 10.1111/j.1749-6632.1971.tb13552.x.
7
Cell components in the immune response. IV. Relationships and possible interactions.免疫反应中的细胞成分。IV. 关系及可能的相互作用。
Cell Immunol. 1970 Jul;1(2):196-206. doi: 10.1016/0008-8749(70)90007-9.
8
Increase in intracellular immunoglobulins in the majority of splenic lymphoid cells after primary immunization.初次免疫后多数脾淋巴细胞内免疫球蛋白增加。
Eur J Immunol. 1974 Feb;4(2):138-45. doi: 10.1002/eji.1830040214.
9
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Nat New Biol. 1972 Jan 26;235(56):115-8. doi: 10.1038/newbio235115a0.
10
Letter: T cell-dependent mediator in the immune response.信件：免疫反应中T细胞依赖性介质。
Nature. 1973 Jun 8;243(5406):356-7. doi: 10.1038/243356a0.

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