Tietz H J, Montag T, Brose E, Hiepe T, Mann W, Hiepe F, Halle H
Angew Parasitol. 1986 Nov;27(4):201-6.
Extracts were prepared from macrocysts of Sarcocystis gigantea, an animal parasite, by sonication and high speed centrifugation. Mononuclear cells were obtained from the blood of 14 adults and 5 newborns and used for transformation assays. Addition of Sarcocystis extracts (equivalent to 400 micrograms protein/ml) to these cells increased the lymphocyte proliferation approximately 71 fold over the background. The control with Pokeweed mitogen (5 micrograms/ml) gave a stimulation index of 58. There was no difference in the response to the Sarcocystis extract between the lymphocytes of adults and those of newborns. This findings show that the effect of the extract is mitogenic and not due an anamnestic reaction.
通过超声处理和高速离心从动物寄生虫巨型肉孢子虫的大囊泡中制备提取物。从14名成年人和5名新生儿的血液中获取单核细胞，并用于转化试验。向这些细胞中添加肉孢子虫提取物（相当于400微克蛋白质/毫升）使淋巴细胞增殖比背景水平增加了约71倍。用商陆有丝分裂原（5微克/毫升）作为对照，刺激指数为58。成年人和新生儿的淋巴细胞对肉孢子虫提取物的反应没有差异。这些发现表明提取物的作用是促有丝分裂的，而不是由于回忆反应。
