Tietz H J, Montag T, Brose E, Widera P, Kiessig S T, Mann W, Hiepe T
Institut für Medizinische Mikrobiologie des Bereiches Medizin (Charité), Humboldt-Universität, Berlin.
Parasitol Res. 1990;76(4):332-5. doi: 10.1007/BF00928188.
The present study further examined the in vitro response of human mononuclear cells (MNC) to the Sarcocystis gigantea lectin (SGL). The results confirm our previous report that SGL is mitogenic for human MNC. We now report that SGL is not only a potent mitogen but also a polyclonal activator for human peripheral B cells. As was true for pokeweed mitogen (PWM, 2 micrograms/ml), the addition of SGL (25 micrograms protein/ml) to cultures of MNC caused lymphocyte proliferation and B-cell maturation, indicated by a marked increase in IgG and IgM production. As measured by the [3H]-thymidine incorporation assay, SGL induced significantly higher proliferative responses than PWM (P less than 0.01, n = 24). The values obtained by SGL and PWM for IgG and IgM synthesis were essentially identical. As opposed to SGL, the sarcotoxin-containing fraction (SGTF) did not induce antibody formation or proliferative responses in human MNC.
本研究进一步检测了人单核细胞（MNC）对巨型肉孢子虫凝集素（SGL）的体外反应。结果证实了我们之前的报告，即SGL对人MNC具有促有丝分裂作用。我们现在报告，SGL不仅是一种有效的促有丝分裂原，也是人外周血B细胞的多克隆激活剂。与商陆有丝分裂原（PWM，2微克/毫升）的情况一样，向MNC培养物中添加SGL（25微克蛋白质/毫升）会导致淋巴细胞增殖和B细胞成熟，这表现为IgG和IgM产生显著增加。通过[3H] - 胸腺嘧啶核苷掺入试验测量，SGL诱导的增殖反应明显高于PWM（P < 0.01，n = 24）。SGL和PWM获得的IgG和IgM合成值基本相同。与SGL相反，含肌毒素的部分（SGTF）在人MNC中未诱导抗体形成或增殖反应。
