Drössigk V U, Tietz H J, Pötzsch F, Scholz D, Gantenberg R, Hiepe T
Institut für Parasitologie und Tropenveterinärmedizin Freien Universität Berlin.
Berl Munch Tierarztl Wochenschr. 1996 Feb;109(2):41-5.
Basis of this study were the previous findings regarding isolation and characterization of a Sarcocystis gigantea lectin (SGL) especially the activation of human mononuclear cells (CD3+, CD4+, CD8+, Mø, B-lymphocytes). HIV-susceptible, immortaliced cell lines (H9-, MT-4) should be investigated to examine their reactivity against SGE which contains this strong mitogen. Using lymphocyte proliferation assay a strong stimulation of noninfected CD(4+)-positive H9-cell by SGE was observed. HIV-infected H(9+)-cells showed after SGE stimulation (20-160 micrograms) an exacerbation with an optimum at day 4. The virus replication in the H(9+)-hostcells was 13 times stronger. This result could also be detected indirectly because of the higher cytotoxicity in the MT-4 cell system. Cytopathogeny was measured by MTT cellvitality assay. In parallel, the high sensitive p24 core Profile-ELISA was used to directly examine the amount of produced HIV. In case of the H(9+)-cells the virus release per cell was 5 times higher after SGE stimulation compared with control.
本研究的基础是先前关于巨型肉孢子虫凝集素（SGL）的分离和特性鉴定的研究结果，尤其是对人单核细胞（CD3 +、CD4 +、CD8 +、Mø、B淋巴细胞）的激活作用。应研究对HIV敏感的永生细胞系（H9 -、MT - 4），以检测它们对含有这种强有丝分裂原的巨型肉孢子虫提取物（SGE）的反应性。使用淋巴细胞增殖试验，观察到SGE对未感染的CD(4 +)阳性H9细胞有强烈刺激作用。SGE刺激（20 - 160微克）后，HIV感染的H(9 +)细胞在第4天出现加剧，病毒复制在H(9 +)宿主细胞中增强了13倍。由于MT - 4细胞系统中细胞毒性较高，这一结果也可间接检测到。通过MTT细胞活力测定法测量细胞病变作用。同时，使用高灵敏度的p24核心Profile - ELISA直接检测产生的HIV量。对于H(9 +)细胞，SGE刺激后每个细胞的病毒释放量比对照高5倍。
