Ding Shuangcheng, Feng Xin, Du Hewei, Wang Hongwei
Agricultural College, Yangtze University, Jingzhou, China.
Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou, China.
PeerJ. 2019 Apr 11;7:e6765. doi: 10.7717/peerj.6765. eCollection 2019.
Worldwide cultivation of maize is often impacted negatively by drought stress. Hyperosmolality-gated calcium-permeable channels (OSCA) have been characterized as osmosensors in . However, the involvement of members of the maize () gene family in response to drought stress is unknown. It is furthermore unclear which gene plays a major role in genetic improvement of drought tolerance in Maize.
We predicted the protein domain structure and transmembrane regions by using the NCBI Conserved Domain Database database and TMHMM server separately. The phylogeny tree was built by Mega7. We used the mixed linear model in TASSEL to perform the family-based association analysis.
In this report, 12 genes were uncovered in the maize genome by a genome-wide survey and analyzed systematically to reveal their synteny and phylogenetic relationship with the genomes of rice, maize, and sorghum. These analyses indicated a relatively conserved evolutionary history of the gene family. Protein domain and transmembrane analysis indicated that most of the 12 ZmOSCAs shared similar structures with their homologs. The result of differential expression analysis under drought at various stages, as well as the expression profiles in 15 tissues, revealed a functional divergence of genes. Notably, the expression level of being up-regulated in both seedlings and adult leaves. Notably, the association analysis between genetic variations in these genes and drought tolerance was detected. Significant associations between genetic variation in and drought tolerance were found at the seedling stage. Our report provides a detailed analysis of the in the maize genome. These findings will contribute to future studies on the functional characterization of ZmOSCA proteins in response to water deficit stress, as well as understanding the mechanism of genetic variation in drought tolerance in maize.
全球范围内玉米种植常受到干旱胁迫的负面影响。超渗透压门控钙通透性通道（OSCA）已被鉴定为[具体物种]中的渗透感受器。然而，玉米（）基因家族成员在干旱胁迫响应中的作用尚不清楚。此外，尚不清楚哪个基因在玉米耐旱性的遗传改良中起主要作用。
我们分别使用NCBI保守结构域数据库和TMHMM服务器预测蛋白质结构域和跨膜区域。用Mega7构建系统发育树。我们在TASSEL中使用混合线性模型进行基于家系的关联分析。
在本报告中，通过全基因组调查在玉米基因组中发现了12个基因，并对其进行了系统分析，以揭示它们与水稻、玉米和高粱基因组的同线性和系统发育关系。这些分析表明该基因家族具有相对保守的进化历史。蛋白质结构域和跨膜分析表明，12个ZmOSCA中的大多数与其同源物具有相似的结构。不同发育阶段干旱胁迫下的差异表达分析结果以及15个组织中的表达谱揭示了基因的功能差异。值得注意的是，在幼苗和成年叶片中均上调。值得注意的是，检测到这些基因的遗传变异与耐旱性之间的关联分析。在幼苗期发现与耐旱性之间的遗传变异存在显著关联。我们的报告提供了对玉米基因组中[该基因家族名称]的详细分析。这些发现将有助于未来对ZmOSCA蛋白在水分亏缺胁迫响应中的功能特性进行研究，以及理解玉米耐旱性遗传变异的机制。
