Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Agricultural College, Yangtze University, Jingzhou 434000, China.
Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Agricultural College, Yangtze University, Jingzhou 434000, China.
Genes (Basel). 2019 Aug 12;10(8):610. doi: 10.3390/genes10080610.
Global maize cultivation is often adversely affected by drought stress. The CC-type glutaredoxin (GRX) genes form a plant-specific subfamily that regulate plant growth and respond to environmental stresses. However, how maize CC-type GRX (ZmGRXCC) genes respond to drought stress remains unclear. We performed a TBLASTN search to identify ZmGRXCCs in the maize genome and verified the identified sequences using the NCBI conservative domain database (CDD). We further established a phylogenetic tree using Mega7 and surveyed known cis-elements in the promoters of ZmGRXCCs using the PlantCARE database. We found twenty-one ZmGRXCCs in the maize genome by a genome-wide investigation and compared their phylogenetic relationships with rice, maize, and Arabidopsis. The analysis of their redox active sites showed that most of the 21 ZmGRXCCs share similar structures with their homologs. We assessed their expression at young seedlings and adult leaves under drought stress and their expression profiles in 15 tissues, and found that they were differentially expressed, indicating that different ZmGRXCC genes have different functions. Notably, ZmGRXCC14 is up-regulated at seedling, V12, V14, V16, and R1 stages. Importantly, significant associations between genetic variation in ZmGRXCC14 and drought tolerance are found at the seedling stage. These results will help to advance the study of the function of ZmGRXCCs genes under drought stress and understand the mechanism of drought resistance in maize.
全球范围内玉米种植常受到干旱胁迫的不利影响。CC 型谷氧还蛋白（GRX）基因构成了一个植物特异性亚家族，调控植物生长并响应环境胁迫。然而，玉米 CC 型 GRX（ZmGRXCC）基因如何响应干旱胁迫尚不清楚。我们通过 TBLASTN 搜索鉴定了玉米基因组中的 ZmGRXCC，并使用 NCBI 保守结构域数据库（CDD）对鉴定的序列进行了验证。我们使用 Mega7 构建了一个系统发育树，并使用 PlantCARE 数据库调查了 ZmGRXCC 启动子中的已知顺式作用元件。通过全基因组研究，我们在玉米基因组中发现了二十一个 ZmGRXCC，并比较了它们与水稻、玉米和拟南芥的系统发育关系。对其氧化还原活性位点的分析表明，21 个 ZmGRXCC 中的大多数与它们的同源物具有相似的结构。我们评估了它们在干旱胁迫下幼叶和成熟叶片中的表达情况以及在 15 种组织中的表达谱，发现它们存在差异表达，表明不同的 ZmGRXCC 基因具有不同的功能。值得注意的是，ZmGRXCC14 在幼苗、V12、V14、V16 和 R1 期上调表达。重要的是，在幼苗期发现 ZmGRXCC14 遗传变异与耐旱性之间存在显著关联。这些结果将有助于推进干旱胁迫下 ZmGRXCC 基因功能的研究，进而理解玉米抗旱的机制。
