Nazeri Mehdi, Salmani Arani Javad, Ziloochi Narjes, Delkhah Hasan, Hesami Arani Mohsen, Asgari Esrafil, Hosseini Mona
Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Department of Environmental Health Engineering, Deputy of Health, Kashan University of Medical Sciences, Kashan, Iran.
MethodsX. 2019 Mar 28;6:666-671. doi: 10.1016/j.mex.2019.03.022. eCollection 2019.
Microbial contamination of computer keyboards and inanimate surfaces of electronic equipment in ICU (Intensive Care Units) can have a significant role for ICU-acquired colonization and a spectrum of nosocomial infections. The aim of this study was to survey the incidence of bacterial contamination and the distribution of species of computer keyboards and inanimate surfaces of bed side equipment in ICUs in Kashan University of medical sciences and health service hospitals. This descriptive, cross-sectional study was done on 75 computer keyboards and inanimate surfaces electronic equipment in 5 ICUs during 2016-2017. Samples were collected from computer keyboards and electronic equipment with normal saline rinsed swabs. Samples were Cultivated on Blood Agar (BA), and MacConkey Agar (MAC) and growing bacteria were identified based on their morphology and biochemical properties. Seventy six (76%) out of 75 computer Keyboards and electronic equipment were contaminated with bacteria and fungi. The most contamination pertained to gram positive bacteria (70.7%) and the most isolated bacteria were coagulase-negative . The highest contamination rates were found on computer keyboards and electronic equipment of which were nurses. •This study demonstrates that monitoring inanimate surfaces and considering these surfaces as source of nosocomial infections is necessary.•In total, Seventy six (76%) out of 75 computer keyboards and electronic equipment in ICUs had positive culture.•It can be concluded that it is necessary for ICUs of Kashan university of medical sciences and Health service hospitals to have practical and regular program to reduce nosocomial infections.
重症监护病房（ICU）中计算机键盘及电子设备无生命表面的微生物污染，在ICU获得性定植及一系列医院感染中可能起重要作用。本研究旨在调查喀山医科大学及健康服务医院ICU中计算机键盘及床边设备无生命表面的细菌污染发生率及菌种分布。这项描述性横断面研究于2016 - 2017年期间在5个ICU中的75个计算机键盘及无生命表面电子设备上进行。用生理盐水冲洗拭子从计算机键盘和电子设备上采集样本。样本接种于血琼脂（BA）和麦康凯琼脂（MAC）上，根据细菌形态和生化特性鉴定生长的细菌。75个计算机键盘和电子设备中有76个（76%）被细菌和真菌污染。污染最多的是革兰氏阳性菌（70.7%），最常分离出的细菌是凝固酶阴性菌。在护士使用的计算机键盘和电子设备上发现的污染率最高。•本研究表明，监测无生命表面并将这些表面视为医院感染源是必要的。•总的来说，ICU中75个计算机键盘和电子设备中有76个（76%）培养呈阳性。•可以得出结论，喀山医科大学及健康服务医院的ICU有必要制定切实可行的常规计划以减少医院感染。
