Chen Jin-Cui, Gong Ya-Jun, Shi Pan, Wang Ze-Hua, Cao Li-Jun, Wang Peng, Wei Shu-Jun
Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguanghuayuan Middle Road, Haidian District, Beijing, 100097, China.
Dow AgroSciences (China) Co., Ltd., Shanghai Branch, Shanghai, 201203, China.
Exp Appl Acarol. 2019 Apr;77(4):545-554. doi: 10.1007/s10493-019-00359-3. Epub 2019 Apr 17.
The acaricide bifenazate acts as complex III inhibitor whereas cyenopyrafen and SYP-9625 act as complex II inhibitors. All these acaricides are commonly used to control two-spotted spider mite (TSSM), Tetranychus urticae Koch. We examined field-evolved and laboratory-selected resistance of TSSM to these three acaricides and determined cross-resistance among them. Six field populations of TSSM showed low levels of resistance to bifenazate with resistance ratios ranging from 2.20 to 10.65 compared to a susceptible strain. SYP-9625, structurally similar to cyenopyrafen, showed slightly higher activity to TSSMs but significant cross-resistance in both field populations and a laboratory-selected strain by SYP-9625. However, low levels of resistance to these two chemicals were found in field populations even when used for short time periods. Cross-resistance was not found between bifenazate and Complex II inhibitors, cyenopyrafen and SYP-9625, in both field populations and the laboratory-selected strain. Field-evolved resistance of TSSM to the tested acaricides is still low and should be delayed by the implementation of resistance management practices. Cross-resistance between cyenopyrafen and SYP-9625 is obvious, so they should not be used together in resistance management strategies based on mode of action rotation.
杀螨剂联苯肼酯作为复合物III抑制剂，而乙螨唑和SYP-9625作为复合物II抑制剂。所有这些杀螨剂通常用于防治二斑叶螨（TSSM），即二斑叶螨（Tetranychus urticae Koch）。我们研究了二斑叶螨对这三种杀螨剂的田间进化抗性和实验室筛选抗性，并确定了它们之间的交叉抗性。与敏感品系相比，六个二斑叶螨田间种群对联苯肼酯表现出低水平抗性，抗性倍数在2.20至10.65之间。与乙螨唑结构相似的SYP-9625对二斑叶螨表现出略高的活性，但在田间种群和SYP-9625实验室筛选品系中均存在显著的交叉抗性。然而，即使在短时间内使用，田间种群对这两种化学药剂的抗性水平也较低。在田间种群和实验室筛选品系中，联苯肼酯与复合物II抑制剂乙螨唑和SYP-9625之间均未发现交叉抗性。二斑叶螨对所测试杀螨剂的田间进化抗性仍然较低，应通过实施抗性管理措施来延缓抗性发展。乙螨唑和SYP-9625之间的交叉抗性明显，因此在基于作用方式轮换的抗性管理策略中，它们不应一起使用。
