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[新生犊牛免疫球蛋白G供应量的测定]

[Determination of the immunoglobulin G supply in the newborn calf].

作者信息

Hampe Marian, Wehrend Axel

机构信息

Klinik für Geburtshilfe, Gynäkologie und Andrologie der Groß- und Kleintiere der Justus-Liebig-Universität Gießen.

出版信息

Tierarztl Prax Ausg G Grosstiere Nutztiere. 2019 Apr;47(2):97-109. doi: 10.1055/a-0858-4038. Epub 2019 Apr 18.

DOI:10.1055/a-0858-4038
PMID:30999350
Abstract

A sufficient supply of colostral antibodies within the first hours of life is crucial for the development and the health status in young calves. It is rational to examine the immunoglobulin uptake of single animals, but particularly on a herd basis, during herd controls and consultations. This enables economical calf rearing in accordance with animal welfare. Because of the costly, laboratory-dependent and in part time-consuming direct measurement of the absorbed immunoglobulins using radial immunodiffusion (RID) or ELISA, multiple studies attempted to develop indirect methods, which would be affordable and operational in the field. These aim to draw an inference for the absorbed quantity of colostral antibodies based on other correlated parameters. Multiple validations showed in part significant differences between various methods concerning specificity and sensitivity in comparison to the direct methods. In addition to RID and ELISA, this article presents the measurement of the γ-glutamyltransferase (GGT) activity, the determination of the total serum protein concentration using refractometry and the zinc sulphate turbidity test, and describes the advantages and disadvantages of their application. Refractory measurement and determination of the GGT activity represent a valuable alternative to a laboratory-dependent immunoglobulin G measurement. Nevertheless, there is no ideal rapid test method, such that several influencing factors have to be considered.

摘要

在犊牛出生后的最初几个小时内,充足的初乳抗体供应对其发育和健康状况至关重要。在畜群检查和咨询期间,检查单头动物的免疫球蛋白摄取情况是合理的,尤其是在畜群层面。这有助于在符合动物福利的前提下实现经济的犊牛饲养。由于使用放射免疫扩散(RID)或酶联免疫吸附测定(ELISA)直接测量吸收的免疫球蛋白成本高昂、依赖实验室且部分耗时,多项研究试图开发间接方法,这些方法在现场应是经济可行的。这些方法旨在根据其他相关参数推断初乳抗体的吸收量。多项验证表明,与直接方法相比,各种方法在特异性和敏感性方面部分存在显著差异。除了RID和ELISA,本文还介绍了γ-谷氨酰转移酶(GGT)活性的测定、使用折射法测定血清总蛋白浓度以及硫酸锌浊度试验,并描述了它们应用的优缺点。GGT活性的难熔测量和测定是依赖实验室的免疫球蛋白G测量的一种有价值的替代方法。然而,如果要考虑几个影响因素,就没有理想的快速检测方法。

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[Determination of the immunoglobulin G supply in the newborn calf].[新生犊牛免疫球蛋白G供应量的测定]
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