School of Agriculture, Yunnan University, Kunming, Yunnan 650091, China.
Biocontrol Engineering Research Center of Plant Disease & Pest, Yunnan University, Kunming 650504, China.
Molecules. 2019 Apr 17;24(8):1514. doi: 10.3390/molecules24081514.
Melatonin can increase plant resistance to stress, and exogenous melatonin has been reported to promote stress resistance in plants. In this study, a melatonin biosynthesis-related gene was cloned from tomato ( Mill. cv. ), which is highly expressed in fruits compared with other organs. The protein was found to locate in the cytoplasm. Melatonin content in overexpression transgenic tomato plants was significantly higher than that in wild-type plants. Under 800 mM NaCl stress, the transcript level of in tomato leaf was positively related to the melatonin contents. Furthermore, compared with that in wild-type plants, levels of superoxide and hydrogen peroxide were lower while the content of proline was higher in transgenic tomatoes. Therefore, was closely associated with melatonin biosynthesis confers the significant salt tolerance, providing a clue to cope with the growing global problem of salination in agricultural production.
褪黑素可以提高植物的抗逆性，已有报道称外源性褪黑素可以促进植物的抗逆性。本研究从番茄（Mill. cv.）中克隆了一个与褪黑素生物合成相关的基因，该基因在果实中的表达水平明显高于其他器官。该蛋白被发现定位于细胞质中。过表达转基因番茄植株中的褪黑素含量明显高于野生型植株。在 800mM NaCl 胁迫下，番茄叶片中 基因的转录水平与褪黑素含量呈正相关。此外，与野生型植株相比，转基因番茄中超氧化物和过氧化氢的水平较低，脯氨酸的含量较高。因此，与褪黑素生物合成密切相关的 基因赋予了番茄显著的耐盐性，为应对农业生产中日益严重的盐渍化问题提供了线索。
