记忆缺陷与雄性大鼠海马体中镁和锰的反常调节有关。
Deficit in Memory Is Associated with Paradoxical Regulation of Magnesium and Manganese in Isolated Male Rats.
作者信息
Famitafreshi Hamidreza, Karimian Morteza
机构信息
Department of Physiology, Tehran University of Medical Science-International Campus, Tehran, Iran.
Department of Physiology, Tehran University of Medical Science, Tehran, Iran.
出版信息
Ann Neurosci. 2018 Dec;25(4):241-246. doi: 10.1159/000491918. Epub 2018 Aug 23.
BACKGROUND
Magnesium (Mg) and manganese (Mn) play an important role in brain development and glutathione, the master antioxidant in the brain, is necessary for the cognitive function. This study is aimed at determining the effect of magnesium and manganese concentrations in hippocampus and prefrontal cortex on the glutathione function. The reduction in glutathione leads to memory impairment in -Y-maze in the isolation period.
METHODS
In this study, 16 male Sprague-Dawley rats were randomly divided into 2 groups: social and isolation. Based on previous studies, the isolation period was considered as 14 days, plus 1 week for acclimatization. On day 15, after assessing the memory with Y-maze, rats were examined for glutathione in serum, hippocampus, and prefrontal cortex. Also, serum, hippocampus, and prefrontal cortex were obtained for assessing Mn and Mg.
RESULTS
The memory in Y-maze was impaired in isolated rats. Also, glutathione in hippocampus and prefrontal cortex was reduced in isolated rats versus socialized rats. In serum assay, glutathione reduced in socialized rats versus isolated rats. Mg was reduced in isolated rats versus socialized rats in serum assay. Paradoxically, Mn was increased in isolated rats versus socialized rats in serum assay. In tissue assay, Mg in hippocampus increased in socialized rats versus isolated rats. Paradoxically, in prefrontal cortex Mg increased in isolated rats versus socialized rats. Mn in hippocampus showed increased concentration in socialized rats versus isolated rats. Paradoxically, Mn showed reduced concentration in prefrontal cortex in socialized rats versus isolated rats.
CONCLUSION
Manganese and magnesium are necessary elements for maintaining enough level of cognition in the isolation period. Also, glutathione plays an important role.
背景
镁（Mg）和锰（Mn）在大脑发育中发挥重要作用，而谷胱甘肽作为大脑中的主要抗氧化剂，对认知功能至关重要。本研究旨在确定海马体和前额叶皮质中镁和锰的浓度对谷胱甘肽功能的影响。在隔离期，谷胱甘肽的减少会导致在Y迷宫中出现记忆障碍。
方法
在本研究中，16只雄性Sprague-Dawley大鼠被随机分为两组：群居组和隔离组。根据先前的研究，隔离期为14天，外加1周的适应期。在第15天，用Y迷宫评估记忆后，检测大鼠血清、海马体和前额叶皮质中的谷胱甘肽。此外，获取血清、海马体和前额叶皮质以评估锰和镁。
结果
隔离大鼠在Y迷宫中的记忆受损。此外，与群居大鼠相比，隔离大鼠海马体和前额叶皮质中的谷胱甘肽减少。在血清检测中，群居大鼠的谷胱甘肽比隔离大鼠减少。在血清检测中，与群居大鼠相比，隔离大鼠的镁减少。矛盾的是，在血清检测中，与群居大鼠相比，隔离大鼠的锰增加。在组织检测中，与隔离大鼠相比，群居大鼠海马体中的镁增加。矛盾的是，与群居大鼠相比，隔离大鼠前额叶皮质中的镁增加。与隔离大鼠相比，群居大鼠海马体中的锰浓度增加。矛盾的是，与隔离大鼠相比，群居大鼠前额叶皮质中的锰浓度降低。
结论
锰和镁是在隔离期维持足够认知水平的必需元素。此外，谷胱甘肽发挥着重要作用。
Zotero 插件