Yusree Farhana Iylia Fatinee Mohd, Peter Angela Paul, Mohd Nor Mohd Zuhair, Show Pau Loke, Mokhtar Mohd Noriznan
Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, Serdang 43400, Malaysia.
Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Semenyih 43400, Malaysia.
Foods. 2021 Nov 9;10(11):2748. doi: 10.3390/foods10112748.
In recent years, downstream bioprocessing industries are venturing into less tedious, simple, and high-efficiency separation by implementing advanced purification and extraction methods. This review discusses the separation of proteins, with the main focus on amylase as an enzyme from agricultural waste using conventional and advanced techniques of extraction and purification via a liquid biphasic system (LBS). In comparison to other methods, such as membrane extraction, precipitation, ultrasonication, and chromatography, the LBS stands out as an efficient, cost-effective, and adaptable developing method for protein recovery. The two-phase separation method can be water-soluble polymers, or polymer and salt, or alcohol and salt, which is a simpler and lower-cost method that can be used at a larger purification scale. The comparison of different approaches in LBS for amylase purification from agricultural waste is also included. Current technology has evolved from a simple LBS into microwave-assisted LBS, liquid biphasic flotation (LBF), thermoseparation (TMP), three-phase partitioning (TPP), ultrasound-assisted LBS, and electrically assisted LBS. pH, time, temperature, and concentration are some of the significant research parameters considered in the review of advanced techniques.
近年来，下游生物加工行业正通过采用先进的纯化和提取方法，探索更简便、高效的分离方式。本综述讨论了蛋白质的分离，主要聚焦于利用双水相系统（LBS）的常规和先进提取及纯化技术，从农业废弃物中分离淀粉酶这种酶。与膜萃取、沉淀、超声处理和色谱法等其他方法相比，双水相系统作为一种高效、经济且适应性强的蛋白质回收开发方法脱颖而出。双相分离方法可以是水溶性聚合物、聚合物与盐或醇与盐，这是一种更简单、成本更低的方法，可用于更大规模的纯化。文中还比较了双水相系统中从农业废弃物中纯化淀粉酶的不同方法。当前技术已从简单的双水相系统发展到微波辅助双水相系统、双相浮选（LBF）、热分离（TMP）、三相分离（TPP）、超声辅助双水相系统和电辅助双水相系统。pH值、时间、温度和浓度是先进技术综述中考虑的一些重要研究参数。
