鼻咽人博卡病毒 1 基因组负荷与腺样体/扁桃体切除术患者的 mRNA 检测或血清学检测无相关性。

No Correlation Between Nasopharyngeal Human Bocavirus 1 Genome Load and mRNA Detection or Serology in Adeno-/Tonsillectomy Patients.

机构信息

Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital and University of Turku, Finland.

Department of Laboratory Medicine, Norwegian University of Science and Technology, Trondheim, Norway.

出版信息

J Infect Dis. 2019 Jul 19;220(4):589-593. doi: 10.1093/infdis/jiz166.

DOI:10.1093/infdis/jiz166

PMID:31001641

Abstract

Human bocavirus 1 (HBoV1) can persist in nasopharynx and tonsils. Using HBoV1 serology, reverse-transcription polymerase chain reaction (PCR) for detecting messenger RNA (mRNA) and quantitative PCR for HBoV1 genome load count, we studied to what extent the HBoV1 DNA loads in nasopharynx correlate with acute infection markers. Tonsillar tissue, nasopharyngeal aspirate, and serum were obtained from 188 elective adeno-/tonsillectomy patients. Relatively high loads of HBoV1 DNA were detected in the nasopharynx of 14 (7%) primarily asymptomatic subjects with negative mRNA and/or serodiagnostic results. Quantitative HBoV1 DNA PCR may have lower specificity than HBoV1 mRNA detection for diagnosing symptomatic infection.

摘要

人博卡病毒 1（HBoV1）可在鼻咽部和扁桃体持续存在。本研究采用 HBoV1 血清学、逆转录聚合酶链反应（PCR）检测信使 RNA（mRNA）和定量 PCR 检测 HBoV1 基因组负荷计数，旨在研究 HBoV1 在鼻咽部的 DNA 负荷与急性感染标志物的相关性。本研究采集了 188 例择期行腺样体/扁桃体切除术患者的扁桃体组织、鼻咽抽吸物和血清。在 14 名（7%）无明显症状且 mRNA 和/或血清学诊断结果均为阴性的患者中，鼻咽部 HBoV1 DNA 负荷相对较高。定量 HBoV1 DNA PCR 检测诊断症状性感染的特异性可能低于 HBoV1 mRNA 检测。