Proenca-Modena J L, Paula F E, Buzatto G P, Carenzi L R, Saturno T H, Prates M C, Silva M L, Delcaro L S, Valera F C P, Tamashiro E, Anselmo-Lima W T, Arruda E
Department of Cell Biology, University of São Paulo School of Medicine, Ribeirão Preto, Brazil Virology Research Center, University of São Paulo School of Medicine, Ribeirão Preto, Brazil Department of Genetics, Evolution, and Bioagents, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.
Department of Cell Biology, University of São Paulo School of Medicine, Ribeirão Preto, Brazil Virology Research Center, University of São Paulo School of Medicine, Ribeirão Preto, Brazil.
J Clin Microbiol. 2014 Aug;52(8):3030-7. doi: 10.1128/JCM.00870-14. Epub 2014 Jun 11.
Human bocavirus 1 (HBoV1) is associated with respiratory infections worldwide, mainly in children. Similar to other parvoviruses, it is believed that HBoV1 can persist for long periods of time in humans, probably through maintaining concatemers of the virus single-stranded DNA genome in the nuclei of infected cells. Recently, HBoV-1 was detected in high rates in adenoid and palatine tonsils samples from patients with chronic adenotonsillar diseases, but nothing is known about the virus replication levels in those tissues. A 3-year prospective hospital-based study was conducted to detect and quantify HBoV1 DNA and mRNAs in samples of the adenoids (AD), palatine tonsils (PT), nasopharyngeal secretions (NPS), and peripheral blood (PB) from patients undergoing tonsillectomy for tonsillar hypertrophy or recurrent tonsillitis. HBoV1 was detected in 25.3% of the AD samples, while the rates of detection in the PT, NPS, and PB samples were 7.2%, 10.5%, and 1.7%, respectively. The viral loads were higher in AD samples, and 27.3% of the patients with HBoV had mRNA detectable in this tissue. High viral loads and detectable mRNA in the AD were associated with HBoV1 detection in the other sample sites. The adenoids are an important site of HBoV1 replication and persistence in children with tonsillar hypertrophy. The adenoids contain high HBoV1 loads and are frequently positive for HBoV mRNA, and this is associated with the detection of HBoV1 in secretions.
人博卡病毒1型（HBoV1）在全球范围内与呼吸道感染有关，主要发生在儿童中。与其他细小病毒类似，人们认为HBoV1能够在人类体内长期持续存在，可能是通过在受感染细胞的细胞核中维持病毒单链DNA基因组的串联体来实现的。最近，在患有慢性腺样体扁桃体疾病患者的腺样体和腭扁桃体样本中检测到HBoV-1的高感染率，但对于该病毒在这些组织中的复制水平却一无所知。我们进行了一项为期3年的基于医院的前瞻性研究，以检测和定量来自因扁桃体肥大或复发性扁桃体炎而接受扁桃体切除术患者的腺样体（AD）、腭扁桃体（PT）、鼻咽分泌物（NPS）和外周血（PB）样本中的HBoV1 DNA和mRNA。在25.3%的AD样本中检测到HBoV1，而在PT、NPS和PB样本中的检测率分别为7.2%、10.5%和1.7%。AD样本中的病毒载量更高，并且27.3%的HBoV患者在该组织中可检测到mRNA。AD中高病毒载量和可检测到的mRNA与其他样本部位检测到HBoV1相关。腺样体是HBoV1在扁桃体肥大儿童中复制和持续存在的重要部位。腺样体含有高HBoV1载量，并且HBoV mRNA经常呈阳性，这与分泌物中检测到HBoV1有关。
