比较儿童急性人博卡病毒 1 感染的表型和基因型诊断。
Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children.
机构信息
Department of Medical Microbiology, Clinic of Laboratory Medicine, St. Olavs Hospital HF, Trondheim University Hospital, 7006, Trondheim, Norway; Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, 7491, Trondheim, Norway.
ArcDia International Oy Ltd, 20521, Turku, Finland.
出版信息
J Clin Virol. 2019 Nov;120:17-19. doi: 10.1016/j.jcv.2019.09.003. Epub 2019 Sep 4.
BACKGROUND
Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA.
OBJECTIVE
This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection.
STUDY DESIGN
HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR.
RESULTS AND CONCLUSION
We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test.
背景
人类博卡病毒 1 型（HBoV1）的诊断一直基于定性 PCR 检测 HBoV1 DNA 或检测 HBoV1 mRNA。
目的
本研究旨在评估快速自动化 HBoV1 抗原检测是否适用于急性 HBoV1 感染的诊断。
研究设计
将 HBoV1 抗原检测与定量 HBoV1 DNA PCR 和 HBoV1 mRNA RT-PCR 进行比较。
结果和结论
我们得出结论，HBoV1 抗原检测的临床特异性和阳性预测值高于 HBoV1 DNA 定性 PCR，但敏感性低于 HBoV1 mRNA 检测。此外，HBoV1 抗原检测具有快速、即时检测的优势。
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