Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
Children's Department, St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway.
J Pediatric Infect Dis Soc. 2023 May 31;12(5):282-289. doi: 10.1093/jpids/piad027.
Human bocavirus 1 (HBoV1) is frequently codetected with other viruses, and detected in asymptomatic children. Thus, the burden of HBoV1 respiratory tract infections (RTI) has been unknown. Using HBoV1-mRNA to indicate true HBoV1 RTI, we assessed the burden of HBoV1 in hospitalized children and the impact of viral codetections, compared with respiratory syncytial virus (RSV).
Over 11 years, we enrolled 4879 children <16 years old admitted with RTI. Nasopharyngeal aspirates were analyzed with polymerase chain reaction for HBoV1-DNA, HBoV1-mRNA, and 19 other pathogens.
HBoV1-mRNA was detected in 2.7% (130/4850) samples, modestly peaking in autumn and winter. Forty-three percent with HBoV1 mRNA were 12-17 months old, and only 5% were <6 months old. A total of 73.8% had viral codetections. It was more likely to detect HBoV1-mRNA if HBoV1-DNA was detected alone (odds ratio [OR]: 3.9, 95% confidence interval [CI]: 1.7-8.9) or with 1 viral codetection (OR: 1.9, 95% CI: 1.1-3.3), compared to ≥2 codetections. Codetection of severe viruses like RSV had lower odds for HBoV1-mRNA (OR: 0.34, 95% CI: 0.19-0.61). The yearly lower RTI hospitalization rate per 1000 children <5 years was 0.7 for HBoV1-mRNA and 8.7 for RSV.
True HBoV1 RTI is most likely when HBoV1-DNA is detected alone, or with 1 codetected virus. Hospitalization due to HBoV1 LRTI is 10-12 times less common than RSV.
人博卡病毒 1 型（HBoV1）常与其他病毒共同检出，且在无症状儿童中也可检出。因此，HBoV1 呼吸道感染（RTI）的负担尚不清楚。本研究使用 HBoV1-mRNA 来指示真正的 HBoV1 RTI，评估了住院儿童中 HBoV1 的负担，并与呼吸道合胞病毒（RSV）进行了比较。
在超过 11 年的时间里，我们纳入了 4879 名<16 岁因 RTI 住院的儿童。通过聚合酶链反应对鼻咽抽吸物进行 HBoV1-DNA、HBoV1-mRNA 和其他 19 种病原体的分析。
在 4850 个样本中检测到 HBoV1-mRNA，秋季和冬季适度升高。43%的 HBoV1 mRNA 患儿年龄为 12-17 个月，仅 5%的患儿年龄<6 个月。共有 73.8%的患儿存在病毒共检出。与≥2 种病毒共检出相比，单独检出 HBoV1-DNA（比值比 [OR]：3.9，95%置信区间 [CI]：1.7-8.9）或与 1 种病毒共检出（OR：1.9，95%CI：1.1-3.3）时，更可能检出 HBoV1-mRNA。与 RSV 等严重病毒共检出时，HBoV1-mRNA 的检出率较低（OR：0.34，95%CI：0.19-0.61）。<5 岁儿童每年每 1000 人因 HBoV1-mRNA 住院的 RTI 发生率为 0.7，因 RSV 住院的 RTI 发生率为 8.7。
当单独检出 HBoV1-DNA 或与 1 种病毒共检出时，最有可能发生真正的 HBoV1 RTI。HBoV1 下呼吸道感染的住院率比 RSV 低 10-12 倍。
