Trondheim University Hospital, and Institute of Laboratory Medicine, Children’s and Women’s Health, Norwegian University of Science and Technology, Trondheim, Norway.
Emerg Infect Dis. 2013 Apr;19(4):574-80. doi: 10.3201/eid1904.121775.
Human bocavirus 1 (HBoV1) is a parvovirus associated with respiratory tract infections (RTIs) in children, but a causal relation has not yet been confirmed. To develop a qualitative reverse transcription PCR to detect spliced mRNA from HBoV1 and to determine whether HBoV1 mRNA correlated better with RTIs than did HBoV1 DNA, we used samples from HBoV1 DNA-positive children, with and without RTIs, to evaluate the test. A real-time reverse transcription PCR, targeting 2 alternatively spliced mRNAs, was developed. HBoV1 mRNA was detected in nasopharyngeal aspirates from 33 (25%) of 133 children with RTIs but in none of 28 controls (p<0.001). The analytical sensitivity and specificity of the test were good. Our data support the hypothesis that HBoV1 may cause RTIs, and we propose that HBoV1 mRNA could be used with benefit, instead of HBoV1 DNA, as a diagnostic target.
人博卡病毒 1（HBoV1）是一种细小病毒，与儿童呼吸道感染（RTIs）有关，但尚未证实其因果关系。为了开发一种定性逆转录聚合酶链反应（RT-PCR）来检测 HBoV1 的拼接 mRNA，并确定 HBoV1 mRNA 与 RTIs 的相关性是否优于 HBoV1 DNA，我们使用了 HBoV1 DNA 阳性、有和无 RTIs 的儿童样本来评估该检测。我们开发了一种针对 2 种选择性拼接 mRNA 的实时 RT-PCR。在 133 例 RTIs 患儿的鼻咽抽吸物中检测到 33 例（25%）存在 HBoV1 mRNA，但在 28 例对照患儿中均未检测到（p<0.001）。该检测的分析灵敏度和特异性良好。我们的数据支持 HBoV1 可能引起 RTIs 的假说，我们建议使用 HBoV1 mRNA 作为诊断靶点，而不是 HBoV1 DNA，可能会带来好处。
