Forensic DNA Typing Research Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan.
Int J Legal Med. 2020 Mar;134(2):521-522. doi: 10.1007/s00414-019-02030-3. Epub 2019 Apr 17.
This population data pertains to 250 unrelated male residents of the Christian population of Lahore, Pakistan. AmpF/STR®Yfiler PCR amplification kit was utilized for evaluation of 17 Y-chromosomal STRs loci. Ancestral lineages and parameters of forensic importance were examined leading to recognition of 135 unique out of 175 total haplotypes with diversity value of 0.991. All Y-STRs portrayed high discrimination power where DYS385 depicted the maximum value of 0.917. Multidimensional Scaling (MDS) and Analysis of Molecular Variance (AMOVA) were generated using YHRD (Y-Chromosome STR Haplotype Reference Database) tools. A pair-wise genetic distance comparison using Rst, Fst, and associated p values lead to the conclusion that studied Christian community was significantly demarcated from the rest of the global populations.
本人口数据涉及 250 名巴基斯坦拉合尔基督教男性居民,他们彼此没有血缘关系。我们使用 AmpF/STR®Yfiler PCR 扩增试剂盒对 17 个 Y 染色体 STR 基因座进行了评估。通过对祖先谱系和具有法医学重要意义的参数进行分析,在 175 个总单倍型中识别出 135 个独特的单倍型,多样性值为 0.991。所有 Y-STR 均具有较高的区分能力,其中 DYS385 具有最高值 0.917。我们使用 YHRD(Y 染色体 STR 单体型参考数据库)工具生成多维尺度分析(MDS)和分子方差分析(AMOVA)。通过 Rst、Fst 及相关 p 值进行的成对遗传距离比较得出结论,所研究的基督教群体与全球其他人群有明显的区别。