Rubab Aqsa, Shafique Muhammad, Javed Faqeeha, Saleem Samia, Zahra Fatima Tuz, McNevin Dennis, Shahid Ahmad Ali
Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan.
School of Biomedical Sciences, The University of Hong Kong, Pok Fu Lam, Hong Kong.
Sci Rep. 2020 Nov 3;10(1):18960. doi: 10.1038/s41598-020-76016-2.
Phylogenetic relationship and the population structure of 500 individuals from the Christian community of Lahore, Pakistan, were examined based on 15 autosomal short tandem repeats (STRs) using the AmpFℓSTR Identifiler Plus PCR Amplification Kit and our previously published Y-filer kit data (17 Y-STRs) of same samples. A total of 147 alleles were observed in 15 loci and allele 11 at the TPOX locus was the most frequent with frequency value (0.464). The data revealed that the Christian population has unique genetic characteristics with respect to a few unusual alleles and their frequencies relative to the other Pakistani population. Significant deviations from Hardy-Weinberg equilibrium were found at two loci (D13S317, D18S51) after Boneferroni's correction (p ≤ 0.003). The combined power of discrimination, combined power of exclusion and cumulative probability of matching were 0.999999999999999978430815060354, 0.999995039393942 and 2.15692 × 10, respectively. On the bases of genetic distances, PCA, phylogenetic and structure analysis Lahore-Christians appeared genetically more associated to south Asian particularly Indian populations like Tamil, Karnataka, Kerala and Andhra Pradesh than rest of global populations.
基于15个常染色体短串联重复序列（STR），使用AmpFℓSTR Identifiler Plus PCR扩增试剂盒以及我们之前发表的相同样本的Y-filer试剂盒数据（17个Y-STR），对来自巴基斯坦拉合尔基督教社区的500名个体的系统发育关系和群体结构进行了研究。在15个位点共观察到147个等位基因，TPOX位点的等位基因11最为常见，频率值为0.464。数据显示，基督教群体在一些异常等位基因及其频率方面相对于其他巴基斯坦群体具有独特的遗传特征。在进行Bonferroni校正后，在两个位点（D13S317、D18S51）发现显著偏离哈迪-温伯格平衡（p≤0.003）。鉴别力联合概率、排除力联合概率和匹配累积概率分别为0.999999999999999978430815060354、0.999995039393942和2.15692×10。基于遗传距离、主成分分析、系统发育和结构分析，拉合尔基督教群体在基因上似乎与南亚特别是印度群体如泰米尔、卡纳塔克、喀拉拉和安得拉邦的群体比全球其他群体更相关。
