Neurobiology Laboratory, Department of Healthcare Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, Pakistan.
Neurobiology Laboratory, Department of Healthcare Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, Pakistan.
Pharmacol Rep. 2019 Jun;71(3):449-456. doi: 10.1016/j.pharep.2019.02.004. Epub 2019 Feb 8.
Hippocampus and entorhinal cortex are key players of learning and memory. Despite their established role in memory processes, the contribution of muscarinic receptor activity in these brain regions during memory retrieval remains elusive. This study was aimed to assess the role of hippocampal CA1 and medial entorhinal cortex muscarinic receptors in memory retrieval.
Mice were implanted with bilateral cannulas in the hippocampus CA1 and medial entorhinal cortex. After recovery they were trained for Morris water maze test, novel object recognition test and contextual fear conditioning. Scopolamine was infused 10 min prior to retrieval test.
Pre-test scopolamine infusion in hippocampal CA1 and medial entorhinal cortex significantly reduced overall exploration of objects (p<0.001). Similarly, pre-retrieval inactivation dorsal hippocampal CA1 and medial entorhinal cortex muscarinic activity caused significant impairment of spatial and fear memories retrieval (p<0.05).
These findings showed vital role of muscarinic activity in retrieving hippocampal and entorhinal cortex dependent memories and suggest a possible target for treating retrograde amnesia.
海马体和内嗅皮层是学习和记忆的关键部位。尽管它们在记忆过程中具有既定的作用，但在记忆检索过程中，这些脑区的毒蕈碱受体活性的贡献仍然难以捉摸。本研究旨在评估海马体 CA1 和内嗅皮层毒蕈碱受体在记忆检索中的作用。
将双侧套管植入小鼠海马体 CA1 和内嗅皮层。恢复后，它们接受 Morris 水迷宫测试、新物体识别测试和情境性恐惧条件反射测试的训练。在检索测试前 10 分钟输注东莨菪碱。
海马体 CA1 和内嗅皮层的预测试东莨菪碱输注显著减少了物体的整体探索（p<0.001）。同样，在检索前，背侧海马体 CA1 和内嗅皮层毒蕈碱活性的失活导致空间和恐惧记忆的检索显著受损（p<0.05）。
这些发现表明毒蕈碱活性在检索海马体和内嗅皮层依赖性记忆中起着至关重要的作用，并为治疗逆行性遗忘提供了一个可能的靶点。
