次氯酸钠和季铵消毒剂对单核细胞增生李斯特菌的杀灭作用

Destruction of Listeria monocytogenes by Sodium Hypochlorite and Quaternary Ammonium Sanitizers.

作者信息

Mustapha A, Liewen M B

机构信息

Department of Food Science and Technology and Food Processing Center, University of Nebraska, Lincoln, NE 68583-0919.

出版信息

J Food Prot. 1989 May;52(5):306-311. doi: 10.4315/0362-028X-52.5.306.

DOI:10.4315/0362-028X-52.5.306

PMID:31003274

Abstract

The antimicrobial effects of two commonly used dairy plant sanitizers on Listeria monocytogenes ATCC 7644 were studied. The two sanitizers used were commercial sodium hypochlorite and quaternary ammonium compound (QAC). The effects were studied on L. monocytogenes in vitro and on stainless steel chips inoculated with the organism. Cells were exposed to concentrations of 0, 50, 100, 200, 400, and 800 ppm chlorine and QAC for 1, 2, and 5 minutes, and neutralized with tryptic soy broth. Decreases in cell numbers ranged from 3-logs to >4-logs in vitro, whereas with the stainless steel, it ranged from 1-log to >4-logs. Scanning electron microscopic (SEM) studies were done to evaluate the attachment characteristics of L. monocytogenes as compared to those of Escherichia coli on stainless steel. L. monocytogenes was found to produce a fibrous-like material similar in appearance to acidic polysaccharide fibrils produced by Pseudomonas sp., which appeared to be removed by the sanitizer solutions.

摘要

研究了两种常用的乳制品厂消毒剂对单核细胞增生李斯特菌ATCC 7644的抗菌效果。所使用的两种消毒剂为市售次氯酸钠和季铵化合物（QAC）。研究了它们对体外单核细胞增生李斯特菌以及接种该菌的不锈钢芯片的作用效果。将细胞暴露于浓度为0、50、100、200、400和800 ppm的氯和QAC中1、2和5分钟，并用胰蛋白胨大豆肉汤进行中和。体外细胞数量减少范围为3个对数至大于4个对数，而在不锈钢上，减少范围为1个对数至大于4个对数。进行了扫描电子显微镜（SEM）研究，以评估与不锈钢上的大肠杆菌相比，单核细胞增生李斯特菌的附着特性。发现单核细胞增生李斯特菌产生一种纤维状物质，其外观类似于假单胞菌属产生的酸性多糖原纤维，而这种物质似乎会被消毒剂溶液去除。