Department of Rehabilitation, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang, 110004, People's Republic of China.
Mol Cell Biochem. 2019 Aug;458(1-2):71-78. doi: 10.1007/s11010-019-03531-w. Epub 2019 Apr 19.
Repetitive transcranial magnetic stimulation (rTMS) is a technique protecting neurons against diverse neurodegenerative disorders by delivering magnetic stimuli into the brain through the intact scalp. In the current study, the protection effect of rTMS on Parkinson's disease (PD) and the associated mechanism driving the treatment were explored. The PD symptoms were induced using 6-OHDA in mice, and the effect of rTMS of two frequencies (1 Hz and 10 Hz) on the cognitive behaviors and neuron viability was detected. Afterwards, the level of Aβ and activity of MKK7-ERK-Fos-APP axis under the administration of rTMS were recorded as well. The intracranial injection of 6-OHDA impaired the cognitive behaviors of the mice in the test of Morris water maze as well as reducing the viability and number of neurons in PD mice. After the treatment of rTMS of both frequencies, the cognitive function of mice was improved and the neuron viability and number were restored in mice brain tissues. The administration of rTMS also increased the cerebrospinal fluid (CSF) level of Aβ in PD mice, which was accompanied by the suppressed levels of p-MKK7, p-ERK1/2, p-c-Fos, and APP. Moreover, the effect of rTMS on mice nerve system was all exerted in a frequency-dependent manner. In conclusion, the findings outlined in the current study affirmed the protection effect of rTMS against PD. The anti-PD function of rTMS was associated with the suppression of MKK7-ERK-Fos-APP axis, which subsequently resulted in the increased CSF Aβ level and decreased brain Aβ level.
重复经颅磁刺激（rTMS）是一种通过完整头皮将磁刺激传递到大脑中来保护神经元免受多种神经退行性疾病影响的技术。在本研究中，探索了 rTMS 对帕金森病（PD）的保护作用及其治疗相关机制。使用 6-OHDA 在小鼠中诱导 PD 症状，并检测 rTMS 两种频率（1 Hz 和 10 Hz）对认知行为和神经元活力的影响。之后，记录 rTMS 作用下 Aβ 水平和 MKK7-ERK-Fos-APP 轴的活性。6-OHDA 颅内注射损害了 Morris 水迷宫测试中小鼠的认知行为，并降低了 PD 小鼠中神经元的活力和数量。两种频率 rTMS 治疗后，改善了小鼠的认知功能，恢复了小鼠脑组织中神经元的活力和数量。rTMS 给药还增加了 PD 小鼠脑脊液（CSF）中的 Aβ 水平，同时降低了 p-MKK7、p-ERK1/2、p-c-Fos 和 APP 的水平。此外，rTMS 对小鼠神经系统的作用均呈频率依赖性。总之，本研究结果证实了 rTMS 对 PD 的保护作用。rTMS 的抗 PD 功能与抑制 MKK7-ERK-Fos-APP 轴有关，从而导致 CSF Aβ 水平升高和脑内 Aβ 水平降低。
