Faculty of Materials, Civil and Environmental Engineering, University of Bielsko-Biała, Bielsko-Biała, Poland.
Faculty of Health Sciences, University of Bielsko-Biała, Bielsko-Biała, Poland.
Mol Cell Biochem. 2019 Aug;458(1-2):125-132. doi: 10.1007/s11010-019-03536-5. Epub 2019 Apr 19.
The basic precondition of proper intrauterine growth is appropriate supply of nutrients transported through placenta. Placenta capacity in the scope of transportation is dependent on transport systems and the structure of the basement membrane and syncytiotrophoblast microvillous membrane. The present pilot study demonstrates preliminary results of the analysis of placenta structure in the course of selected pathologies by FT Raman spectroscopy analysis. The observed changes of the molecular structure in the so-called average spectra, independent of methodical processing, may be an indicator of the efficiency of transportation controlled by syncytiotrophoblast. In particular, an increase in the intensity of dispersion and transfer within the frequency of 3425-3300 cm demonstrate the dynamics of the interaction in the scope of hydrogen bonds in healthy tissues. Changes in the molecular structure within the frequency of 950-750 cm and conformational changes within disulphide bonds differentiate the healthy tissue from the pathological one. Changes in the molecular structure observed in the FTR spectra are a spectroscopic image of placenta functions in the course of various pathologies. They also document a complex goal of our research that is finding spectroscopic biomarkers of regular and pathological placental tissue.
适当的宫内生长的基本前提条件是适当供应通过胎盘运输的营养物质。胎盘在运输范围内的运输能力取决于运输系统以及基底膜和合体滋养层微绒毛膜的结构。本初步研究通过 FT 拉曼光谱分析展示了在选定病理过程中胎盘结构分析的初步结果。在所谓的平均光谱中观察到的分子结构变化，与方法处理无关，可能是由合体滋养层控制的运输效率的指标。特别是，在 3425-3300cm 范围内的分散和转移频率的强度增加表明健康组织中氢键相互作用的动力学。在 950-750cm 范围内的分子结构变化和二硫键内的构象变化将健康组织与病理组织区分开来。在 FTR 光谱中观察到的分子结构变化是各种病理过程中胎盘功能的光谱图像。它们还记录了我们研究的一个复杂目标，即寻找正常和病理性胎盘组织的光谱生物标志物。
