Department of Breast Surgery, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, PR China.
Department of cardiothoracic surgery, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, PR China.
J Biochem Mol Toxicol. 2019 Aug;33(8):e22343. doi: 10.1002/jbt.22343. Epub 2019 Apr 20.
This paper aimed to study the possible involvement of adenosine triphosphate-binding cassette (ABC) transporters in the detoxification of quantum dots (QDs) in human breast carcinoma (SK-BR-3) cells. The effects of QD sizes on such interactions were also evaluated. For this purpose, we used monodispersed MPA-COOH-CdTe QDs with different diameters (emission length at 560 and 625 nm, named as QD-560 and QD-625). Such QDs tended to accumulate in cells and cause significant toxicity. Using specific inhibitors of ABC transporters, the cellular accumulation and toxicity of QDs in SK-BR-3 cells were significantly affected. Moreover, treatment of QDs caused concentration- and time-dependent induction of ABC transporters. Furthermore, the induction effects of smaller QDs were found to be greater than larger ones at equivalent concentrations, suggesting a size-dependent recognition of substrates by ABC transporters. Overall, these results provided important support for the modulation of QDs toxicity by ABC transporters.
本文旨在研究三磷酸腺苷结合盒（ABC）转运蛋白是否参与了人乳腺癌（SK-BR-3）细胞中量子点（QD）的解毒过程。同时还评估了 QD 尺寸对这种相互作用的影响。为此，我们使用了具有不同直径（发射长度为 560nm 和 625nm，分别命名为 QD-560 和 QD-625）的单分散 MPA-COOH-CdTe QD。这些 QD 容易在细胞内积累，导致明显的毒性。使用 ABC 转运蛋白的特异性抑制剂，QD 在 SK-BR-3 细胞中的细胞积累和毒性受到显著影响。此外，QD 的处理导致 ABC 转运蛋白的浓度和时间依赖性诱导。此外，在等效浓度下，较小 QD 的诱导作用大于较大 QD，表明 ABC 转运蛋白对底物具有尺寸依赖性识别。总的来说，这些结果为 ABC 转运蛋白调节 QD 毒性提供了重要依据。
