Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Research Institute of Sciences and New Technology, Mashhad, Iran.
Anal Chim Acta. 2019 Aug 13;1065:107-112. doi: 10.1016/j.aca.2019.02.062. Epub 2019 Mar 18.
Herein, a simple electrochemical sensor is proposed for 17β-estradiol (E2) determination. In this sensing platform, split DNA aptamers for E2 were applied as recognizing agents. In the presence of E2, split aptamers are bound to E2 and establish split1-E2-split2 complex as a bridge on the surface of electrode. This physical bar leads to ultrasensitive detection of E2. This aptasensor is capable of recognizing E2 within 30 min without complicated procedures and expensive equipment. This sensing approach indicated a wide linear range with detection limits of 0.5 pM and 0.7 pM for E2 in tap water and milk samples, respectively. Simplicity and high specificity of the proposed electrochemical aptasensor make it an ideal sensing platform for ultra-low detection of other targets in complicated samples by replacement of split DNA aptamers.
本文提出了一种用于 17β-雌二醇（E2）测定的简单电化学传感器。在这个传感平台中，分裂 DNA 适体被用作识别试剂。在存在 E2 的情况下，分裂适体与 E2 结合，并在电极表面建立作为桥梁的分裂 1-E2-分裂 2 复合物。这种物理障碍导致对 E2 的超灵敏检测。该适体传感器能够在 30 分钟内识别 E2，无需复杂的程序和昂贵的设备。这种传感方法具有较宽的线性范围，对自来水中的 E2 的检测限为 0.5 pM，对牛奶样品中的 E2 的检测限为 0.7 pM。所提出的电化学适体传感器的简单性和高特异性使其成为通过替换分裂 DNA 适体来对复杂样品中其他超低目标进行超灵敏检测的理想传感平台。
