Nanotechnology Innovation Centre, Advanced Materials Division, Mintek, Private Bag X3015, Randburg, Johannesburg 2125, South Africa.
SensorLab (University of the Western Cape Sensor Laboratories), 4th Floor Chemical Sciences Building, University of the Western Cape, Private Bag X17, Bellville, Cape Town 7535, South Africa.
Biosensors (Basel). 2023 Feb 8;13(2):242. doi: 10.3390/bios13020242.
17β-estradiol (E2) is an important natural female hormone that is also classified as an estrogenic endocrine-disrupting compound (e-EDC). It is, however, known to cause more damaging health effects compared to other e-EDCs. Environmental water systems are commonly contaminated with E2 that originates from domestic effluents. The determination of the level of E2 is thus very crucial in both wastewater treatment and in the aspect of environmental pollution management. In this work, an inherent and strong affinity of the estrogen receptor-α (ER-α) for E2 was used as a basis for the development of a biosensor that was highly selective towards E2 determination. A gold disk electrode (AuE) was functionalised with a 3-mercaptopropionic acid-capped tin selenide (SnSe-3MPA) quantum dot to produce a SnSe-3MPA/AuE electroactive sensor platform. The ER-α-based biosensor (ER-α/SnSe-3MPA/AuE) for E2 was produced by the amide chemistry of carboxyl functional groups of SnSe-3MPA quantum dots and the primary amines of ER-α. The ER-α/SnSe-3MPA/AuE receptor-based biosensor exhibited a formal potential (E) value of 217 ± 12 mV, assigned as the redox potential for monitoring the E2 response using square-wave voltammetry (SWV). The response parameters of the receptor-based biosensor for E2 include a dynamic linear range (DLR) value of 1.0-8.0 nM (R = 0.99), a limit of detection (LOD) value of 1.69 nM (S/N = 3), and a sensitivity of 0.04 µA/nM. The biosensor exhibited high selectivity for E2 and good recoveries for E2 determination in milk samples.
17β-雌二醇（E2）是一种重要的天然女性激素，也被归类为雌激素类内分泌干扰化合物（e-EDC）。然而，与其他 e-EDC 相比，它已知会对健康造成更具破坏性的影响。环境水系通常受到 E2 的污染，E2 来源于家庭废水。因此，E2 的水平测定在废水处理和环境污染管理方面都非常关键。在这项工作中，雌激素受体-α（ER-α）对 E2 的固有和强烈亲和力被用作开发对 E2 具有高选择性的生物传感器的基础。金盘电极（AuE）用 3-巯基丙酸封端的锡硒量子点（SnSe-3MPA）功能化，产生 SnSe-3MPA/AuE 电活性传感器平台。基于 ER-α 的生物传感器（ER-α/SnSe-3MPA/AuE）是通过 SnSe-3MPA 量子点的羧基官能团和 ER-α 的伯胺的酰胺化学产生的。ER-α/SnSe-3MPA/AuE 受体基生物传感器的形式电位（E）值为 217±12 mV，被指定为使用方波伏安法（SWV）监测 E2 响应的氧化还原电位。基于受体的生物传感器对 E2 的响应参数包括 1.0-8.0 nM 的动态线性范围（DLR）值（R=0.99）、1.69 nM 的检测限（LOD）值（S/N=3）和 0.04 µA/nM 的灵敏度。该生物传感器对 E2 表现出高选择性，并且对牛奶样品中 E2 的测定具有良好的回收率。
Zotero 插件
