Molinaro G A, Eby W C, Reimer C
J Immunol Methods. 1987 Feb 11;96(2):219-24. doi: 10.1016/0022-1759(87)90317-6.
A monoclonal antibody to human IgG was tested with myeloma proteins of the four IgG subclasses. When tested by immunofluorometric assay, enzyme-linked immunosorbent assay, hemagglutination and hemagglutination inhibition assays, the antibody reacted with IgG3 but not with the other three IgG subclasses. When tested by Ouchterlony assays in the presence of polyethylene glycol, the antibody formed lines with all four IgG proteins. The line with IgG3 was sharp and stable, but the lines with the other three IgG subclasses tended to blur with time and with the lower PEG concentrations. These findings show that Ouchterlony assays can reveal cross-reactions of a monoclonal antibody that can be missed by more sensitive assays.
用一种针对人IgG的单克隆抗体对四种IgG亚类的骨髓瘤蛋白进行检测。当通过免疫荧光测定法、酶联免疫吸附测定法、血凝试验和血凝抑制试验进行检测时，该抗体与IgG3发生反应，但不与其他三种IgG亚类发生反应。当在聚乙二醇存在的情况下通过双向免疫扩散试验进行检测时，该抗体与所有四种IgG蛋白形成沉淀线。与IgG3形成的沉淀线清晰且稳定，但与其他三种IgG亚类形成的沉淀线会随着时间推移以及聚乙二醇浓度降低而趋于模糊。这些发现表明，双向免疫扩散试验能够揭示单克隆抗体的交叉反应，而更灵敏的检测方法可能会遗漏这些交叉反应。
