Molinaro G A, Eby W C
Mol Immunol. 1984 Feb;21(2):181-4. doi: 10.1016/0161-5890(84)90134-2.
A monoclonal antibody reactive with human immunoglobulin (Ig)G4 and a monoclonal antibody reactive with IgG1, IgG2 and IgG4 were tested with an IgG4 myeloma protein by double diffusion in a polyethylene glycol-containing gel. In a three-well Ouchterlony pattern, the IgG4 myeloma protein formed lines of double partial identity (double spur) with the two monoclonal antibodies. The two spurs lengthened and thickened with decreasing concentrations of polyethylene glycol indicating that soluble immune complexes diffused past the precipitin lines and formed the spurs. In a two-well pattern, the myeloma protein formed two lines with mixtures of the two monoclonal antibodies indicating that the immune complexes formed by the two antibodies distributed bimodally in the gel as if two types of complexes were formed. These unpredicted findings indicate that the process of antigen-antibody precipitation in gels needs to be analyzed further by using monoclonal antibodies.
用一种与人免疫球蛋白（Ig）G4反应的单克隆抗体和一种与IgG1、IgG2和IgG4反应的单克隆抗体，在含聚乙二醇的凝胶中通过双向扩散法检测一种IgG4骨髓瘤蛋白。在三孔Ouchterlony模式中，IgG4骨髓瘤蛋白与这两种单克隆抗体形成了双部分同一性线（双刺）。随着聚乙二醇浓度的降低，两个刺变长变粗，表明可溶性免疫复合物扩散穿过沉淀线并形成刺。在两孔模式中，骨髓瘤蛋白与两种单克隆抗体的混合物形成两条线，表明由两种抗体形成的免疫复合物在凝胶中呈双峰分布，就好像形成了两种类型的复合物。这些意外发现表明，需要使用单克隆抗体进一步分析凝胶中抗原-抗体沉淀的过程。
