坐骨神经组织的体内相干反斯托克斯拉曼散射成像
In vivo coherent anti-Stokes Raman scattering imaging of sciatic nerve tissue.
作者信息
Huff T B, Cheng J-X
机构信息
Department of Chemistry,Purdue University, West Lafayette, IN 47907, USA.
出版信息
J Microsc. 2007 Feb;225(Pt 2):175-82. doi: 10.1111/j.1365-2818.2007.01729.x.
Abstract
We report in vivo nonlinear optical imaging of mouse sciatic nerve tissue by epidetected coherent anti-Stokes Raman scattering and second harmonic generation microscopy. Following a minimally invasive surgery to open the skin, coherent anti-Stokes Raman scattering imaging of myelinated axons and second harmonic generation imaging of the surrounding collagen fibres were demonstrated with high signal-to-background ratio, three-dimensional spatial resolution, and no need for labelling. The underlying contrast mechanisms of in vivo coherent anti-Stokes Raman scattering were explored by three-dimensional imaging of fat cells that surround the nerve. The epidetected coherent anti-Stokes Raman scattering signals from the nerve tissues were found to arise from interfaces as well as back reflection of forward coherent anti-Stokes Raman scattering.
摘要
我们报告了通过表皮检测相干反斯托克斯拉曼散射和二次谐波产生显微镜对小鼠坐骨神经组织进行的体内非线性光学成像。在进行微创皮肤切开手术后，展示了有髓轴突的相干反斯托克斯拉曼散射成像以及周围胶原纤维的二次谐波产生成像，具有高信噪比、三维空间分辨率且无需标记。通过对神经周围脂肪细胞的三维成像探索了体内相干反斯托克斯拉曼散射的潜在对比机制。发现来自神经组织的表皮检测相干反斯托克斯拉曼散射信号源于界面以及向前相干反斯托克斯拉曼散射的背向反射。
相似文献
1
In vivo coherent anti-Stokes Raman scattering imaging of sciatic nerve tissue.坐骨神经组织的体内相干反斯托克斯拉曼散射成像
J Microsc. 2007 Feb;225(Pt 2):175-82. doi: 10.1111/j.1365-2818.2007.01729.x.
2
Excitation parameters optimized for coherent anti-Stokes Raman scattering imaging of myelinated tissue.优化用于有髓组织相干反斯托克斯拉曼散射成像的激发参数。
J Biomed Opt. 2019 Apr;24(4):1-8. doi: 10.1117/1.JBO.24.4.046502.
3
Ex vivo and in vivo imaging of myelin fibers in mouse brain by coherent anti-Stokes Raman scattering microscopy.通过相干反斯托克斯拉曼散射显微镜对小鼠脑内髓鞘纤维进行体外和体内成像。
Opt Express. 2008 Nov 24;16(24):19396-409. doi: 10.1364/oe.16.019396.
4
Quantitative myelin imaging with coherent anti-Stokes Raman scattering microscopy: alleviating the excitation polarization dependence with circularly polarized laser beams.利用相干反斯托克斯拉曼散射显微镜进行定量髓鞘成像：用圆偏振激光束减轻激发偏振依赖性。
Opt Express. 2009 Oct 12;17(21):18419-32. doi: 10.1364/OE.17.018419.
5
6
Label-free imaging of lipid dynamics using Coherent Anti-stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) microscopy.无标记脂质动力学成像：相干反斯托克斯拉曼散射（CARS）和受激拉曼散射（SRS）显微镜技术。
Curr Opin Genet Dev. 2011 Oct;21(5):585-90. doi: 10.1016/j.gde.2011.09.003. Epub 2011 Sep 22.
7
RP-CARS: label-free optical readout of the myelin intrinsic healthiness.RP-CARS：髓鞘内在健康状况的无标记光学读出。
Opt Express. 2014 Jun 2;22(11):13733-43. doi: 10.1364/OE.22.013733.
8
Imaging the intact mouse cornea using coherent anti-stokes Raman scattering (CARS).利用相干反斯托克斯拉曼散射（CARS）对完整的小鼠角膜进行成像。
Invest Ophthalmol Vis Sci. 2013 Aug 5;54(8):5258-65. doi: 10.1167/iovs.12-11513.
9
Nonlinear interferometric vibrational imaging.非线性干涉振动成像。
Phys Rev Lett. 2004 Mar 26;92(12):123905. doi: 10.1103/PhysRevLett.92.123905. Epub 2004 Mar 25.
10
Coherent anti-stokes Raman scattering microscopy for high speed non- staining biomolecular imaging.相干反斯托克斯拉曼散射显微镜用于高速非染色生物分子成像。
Curr Pharm Biotechnol. 2013;14(2):150-8.
引用本文的文献
1
Coherent Vibrational Anti-Stokes Raman Spectroscopy Assisted by Pulse Shaping.脉冲整形辅助的相干振动反斯托克斯拉曼光谱
Molecules. 2025 May 21;30(10):2243. doi: 10.3390/molecules30102243.
2
Vibrational spectroscopy and multiphoton microscopy for label-free visualization of nervous system degeneration and regeneration.振动光谱和多光子显微镜用于神经系统退变和再生的无标记可视化
Biophys Rev. 2023 Oct 5;16(2):219-235. doi: 10.1007/s12551-023-01158-2. eCollection 2024 Apr.
3
Label-free intraoperative nerve detection and visualization using ratiometric diffuse reflectance spectroscopy.利用比率型漫反射光谱技术进行无标记术中神经检测和可视化。
Sci Rep. 2023 May 10;13(1):7599. doi: 10.1038/s41598-023-34054-6.
4
Dual-wavelength multimodal multiphoton microscope with SMA-based depth scanning.基于SMA的深度扫描双波长多模态多光子显微镜。
Biomed Opt Express. 2022 Apr 11;13(5):2754-2771. doi: 10.1364/BOE.456390. eCollection 2022 May 1.
5
Imaging Arm Regeneration: Label-Free Multiphoton Microscopy to Dissect the Process in .成像手臂再生：用于剖析该过程的无标记多光子显微镜技术
Front Cell Dev Biol. 2022 Feb 4;10:814746. doi: 10.3389/fcell.2022.814746. eCollection 2022.
6
Eosinophils and Neutrophils-Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy.嗜酸性粒细胞和中性粒细胞的自发拉曼、CARS 和荧光显微镜分子差异。
Cells. 2020 Sep 7;9(9):2041. doi: 10.3390/cells9092041.
7
Rise of Raman spectroscopy in neurosurgery: a review.拉曼光谱学在神经外科学中的兴起：综述。
J Biomed Opt. 2020 May;25(5):1-36. doi: 10.1117/1.JBO.25.5.050901.
8
All-fibre supercontinuum laser for multispectral photoacoustic microscopy of lipids in the extended near-infrared region.用于扩展近红外区域脂质多光谱光声显微镜的全光纤超连续谱激光器。
Photoacoustics. 2020 Jan 27;18:100163. doi: 10.1016/j.pacs.2020.100163. eCollection 2020 Jun.
9
Imaging in the repair of peripheral nerve injury.周围神经损伤修复的影像学。
Nanomedicine (Lond). 2019 Oct;14(20):2659-2677. doi: 10.2217/nnm-2019-0115. Epub 2019 Oct 15.
10
Label-free Imaging of Tissue Architecture during Axolotl Peripheral Nerve Regeneration in Comparison to Functional Recovery.比较功能恢复的同时，对蝾螈周围神经再生过程中组织架构进行无标记成像。
Sci Rep. 2019 Sep 2;9(1):12641. doi: 10.1038/s41598-019-49067-3.
本文引用的文献
1
Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration.利用共线配置的相干反斯托克斯拉曼散射显微镜在指纹区进行分子振动成像。
Opt Lett. 2000 Dec 15;25(24):1768-70. doi: 10.1364/ol.25.001768.
2
Polarization coherent anti-Stokes Raman scattering microscopy.偏振相干反斯托克斯拉曼散射显微镜术
Opt Lett. 2001 Sep 1;26(17):1341-3. doi: 10.1364/ol.26.001341.
3
In vivo imaging of the diseased nervous system.患病神经系统的体内成像。
Nat Rev Neurosci. 2006 Jun;7(6):449-63. doi: 10.1038/nrn1905.
4
Broadly tunable dual-wavelength light source for coherent anti-Stokes Raman scattering microscopy.用于相干反斯托克斯拉曼散射显微镜的宽可调谐双波长光源。
Opt Lett. 2006 May 1;31(9):1292-4. doi: 10.1364/ol.31.001292.
5
Deep tissue two-photon microscopy.深部组织双光子显微镜检查
Nat Methods. 2005 Dec;2(12):932-40. doi: 10.1038/nmeth818.
6
Guillain-Barré syndrome.吉兰-巴雷综合征
Lancet. 2005 Nov 5;366(9497):1653-66. doi: 10.1016/S0140-6736(05)67665-9.
7
Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.采用视频速率相干反斯托克斯拉曼散射显微镜对体内组织进行化学成像。
Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16807-12. doi: 10.1073/pnas.0508282102. Epub 2005 Nov 1.
8
In vitro and in vivo two-photon luminescence imaging of single gold nanorods.单根金纳米棒的体外和体内双光子发光成像
Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):15752-6. doi: 10.1073/pnas.0504892102. Epub 2005 Oct 20.
9
Quantitative coherent anti-Stokes Raman scattering imaging of lipid distribution in coexisting domains.共存域中脂质分布的定量相干反斯托克斯拉曼散射成像
Biophys J. 2005 Nov;89(5):3480-90. doi: 10.1529/biophysj.105.065607. Epub 2005 Aug 26.
10
Simultaneous imaging of GFP, CFP and collagen in tumors in vivo using multiphoton microscopy.利用多光子显微镜在体内对肿瘤中的绿色荧光蛋白（GFP）、青色荧光蛋白（CFP）和胶原蛋白进行同步成像。
BMC Biotechnol. 2005 May 23;5:14. doi: 10.1186/1472-6750-5-14.
Zotero 插件