Kochendoerfer Kiara, Sa Gomes Pedro, Rütze Dennis N, Pathapati Trinath, de Wit Pieter, Zaalberg Menne
BASF SE Carl-Bosch-Strasse 38 67056 Ludwigshafen Germany.
Xendo B.V. Bio Science Park, Schipholweg 73-75 2316 ZL Leiden The Netherlands.
Chem Eng Technol. 2018 Dec;41(12):2418-2426. doi: 10.1002/ceat.201800298. Epub 2018 Oct 30.
Complex streams in bio-based industries require efficient downstream processing units. Simulated moving-bed (SMB) chromatography is known to improve process efficiency by reducing resin and buffer requirement, but it can be further enhanced by technology hybridization. In the current experiments, an SMB system has been integrated with a bipolar electrodialysis (BPED) system. SMB purified -aminobutyric acid (GABA) from a clarified fermentation broth while BPED processed the product-containing eluent stream into recyclable eluent and purified product streams. The continuous operation did not result in any impurity accumulation.
生物基产业中的复杂流程需要高效的下游处理单元。已知模拟移动床（SMB）色谱法可通过减少树脂和缓冲液需求来提高工艺效率，但通过技术杂交可进一步提升其性能。在当前实验中，一个SMB系统已与双极电渗析（BPED）系统集成。SMB从澄清的发酵液中纯化γ-氨基丁酸（GABA），而BPED则将含有产物的洗脱液流处理成可循环使用的洗脱液和纯化的产物流。连续运行未导致任何杂质积累。
