1 Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
2 Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Faiyum, Egypt.
J Interferon Cytokine Res. 2019 Jul;39(7):428-437. doi: 10.1089/jir.2019.0001. Epub 2019 Apr 22.
Rheumatoid arthritis (RA) is a chronic immune-mediated inflammatory disease. We aimed to measure the level of miR-155 and its genetic variant rs767649 in patients with RA and to evaluate their relationship with ischemia-modified albumin (IMA). The study was performed on 79 patients with RA (group I) and 78 healthy control participants (group II). Quantitative real-time polymerase chain reaction was used to assess the expression of serum miR-155 in addition to its functional variant rs767649. IMA levels were measured by enzyme-linked immunosorbent assay. Significant overexpression of miR-155 and higher levels of IMA were detected in patients with RA compared with those in controls ( < 0.0001). The fold change in miR-155 was significantly positively associated with IMA ( = 0.362, = 0.001) in patients with RA. Significant differences in the frequency of miR-155 (rs767649) genotypes and alleles were noted between patients with RA and controls. MiR-155 and IMA levels were significantly associated with the genotype distribution of miR-155 (rs767649) in patients with RA and were higher in patients with the TT genotype. MiR-155 and its functional variant rs767649 might play an important role in susceptibility to the increased risk of RA, stressing the role of miR-155 as a therapeutic target in the treatment of RA. In addition, IMA levels were increased and correlated with miR-155 and its single nucleotide polymorphism rs767649 in Egyptian patients with RA.
类风湿关节炎（RA）是一种慢性免疫介导的炎症性疾病。我们旨在测量 RA 患者中 miR-155 的水平及其遗传变异 rs767649，并评估它们与缺血修饰白蛋白（IMA）的关系。该研究在 79 例 RA 患者（I 组）和 78 例健康对照参与者（II 组）中进行。使用实时定量聚合酶链反应来评估血清 miR-155 的表达及其功能变异 rs767649。通过酶联免疫吸附试验测量 IMA 水平。与对照组相比，RA 患者的 miR-155 表达显著上调，IMA 水平也更高（<0.0001）。RA 患者中 miR-155 的倍数变化与 IMA 呈显著正相关（=0.362，=0.001）。在 RA 患者和对照组之间，miR-155（rs767649）基因型和等位基因的频率存在显著差异。miR-155 和 IMA 水平与 RA 患者 miR-155（rs767649）的基因型分布显著相关，且 TT 基因型患者的水平更高。miR-155 及其功能变异 rs767649 可能在易感性增加 RA 风险中发挥重要作用，强调了 miR-155 作为 RA 治疗靶点的作用。此外，埃及 RA 患者的 IMA 水平升高，并与 miR-155 及其单核苷酸多态性 rs767649 相关。
