Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Science, East China Normal University, Shanghai, 200241, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, China; Joint Research Institute for New Energy and the Environment, East China Normal University and Colorado State University, Shanghai, 200062, China.
Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Science, East China Normal University, Shanghai, 200241, China.
Water Res. 2019 Jul 1;158:11-21. doi: 10.1016/j.watres.2019.03.010. Epub 2019 Apr 12.
The widespread of water borne antibiotic resistance genes (ARGs) represents a growing threat to the health of millions of people. Our study detected the relative abundances of 10 ARG subtypes in the Shanghai river network, where the major ARG components were strB, sul1, and ermB. These ARGs were significantly enriched by the combined sewage, tail water from urban wastewater treatment plant and runoff from agricultural areas, which reached the Suzhou (SZ), Dianpu (DP), and Huangpu (HP) River, respectively (one-way ANOVA, P < 0.01). The target ARGs were distributed in varying patterns across different rivers. bla and bla contributed to the increase of total ARGs in the rivers influenced by urban sources, particularly in the SZ River, whose distribution of ARGs was significantly related to that of the confluence of the whole river network (Mantel test, P < 0.01). The bacterial community was closely structured with ARGs and potential pathogenic bacteria's association with target ARGs became significant in downstream samples (Procrustes test, P = 0.03). Water near urban wastewater fallouts was observed to have the highest content of intl1 in the DP River, whose downstream samples' intl -ARG relationship fitted the same regression model as that of the network confluence (R = 0.84, P < 0.001). The amelioration of river water quality does not reduce ARGs, but may affect their distributional patterns in the river network in Shanghai.
水中抗生素耐药基因（ARGs）的广泛传播对数百万人的健康构成了日益严重的威胁。我们的研究检测了上海河网中 10 种 ARG 亚型的相对丰度，其中主要的 ARG 成分是 strB、sul1 和 ermB。这些 ARGs 在综合污水、城市污水处理厂尾水和农业区径流的共同作用下显著富集，分别到达苏州河（SZ）、淀浦河（DP）和黄浦江（HP）（单向方差分析，P<0.01）。目标 ARGs 在不同河流中的分布模式存在差异。bla 和 bla 促进了受城市源影响的河流中总 ARGs 的增加，特别是在 SZ 河，其 ARGs 的分布与整个河网的汇流明显相关（Mantel 检验，P<0.01）。细菌群落与 ARGs 密切相关，下游样本中潜在的致病细菌与目标 ARGs 的关联变得显著（Procrustes 检验，P=0.03）。在 DP 河，靠近城市废水降落处的水观察到 intl1 的含量最高，其下游样本中的 intl-ARG 关系与网络汇流的回归模型相同（R=0.84，P<0.001）。河水水质的改善不会减少 ARGs，但可能会影响上海河网中 ARGs 的分布模式。
