基于银掺杂介孔二氧化硅纳米颗粒的无酶电化学传感器用于测定活细胞释放的过氧化氢。
Silver Doped Mesoporous Silica Nanoparticles Based Electrochemical Enzyme-Less Sensor for Determination of HO Released from Live Cells.
作者信息
Yang Danting, Ni Ning, Cao Lu, Song Xin, Alhamoud Yasmin, Yu Guangxia, Zhao Jinshun, Zhou Haibo
机构信息
Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medical School of Ningbo University, Ningbo 315211, China.
School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
出版信息
Micromachines (Basel). 2019 Apr 21;10(4):268. doi: 10.3390/mi10040268.
In this study, a silver doped mesoporous silica nanoparticles-based enzyme-less electrochemical sensor for the determination of hydrogen peroxide (HO) released from live cells was constructed for the first time. The presented electrochemical sensor exhibited fast response (2 s) towards the reduction of HO concentration variation at an optimized potential of -0.5 V with high selectivity over biological interferents such as uric acid, ascorbic acid, and glucose. In addition, a wide linear range (4 μM to 10 mM) with a low detection limit (LOD) of 3 μM was obtained. Furthermore, the Ag-mSiO nanoparticles/glass carbon electrode (Ag-mSiO NPs/GCE) based enzyme-less sensor showed good electrocatalytic performance, as well as good reproducibility, and long-term stability, which provided a successful way to in situ determine HO released from live cells. It may also be promising to monitor the effect of reactive oxygen species (ROS) production in bacteria against oxidants and antibiotics.
在本研究中，首次构建了一种基于银掺杂介孔二氧化硅纳米颗粒的无酶电化学传感器，用于测定活细胞释放的过氧化氢（HO）。所展示的电化学传感器在-0.5 V的优化电位下，对HO浓度变化的还原表现出快速响应（2秒），对尿酸、抗坏血酸和葡萄糖等生物干扰物具有高选择性。此外，获得了宽线性范围（4 μM至10 mM），检测限（LOD）低至3 μM。此外，基于Ag-mSiO纳米颗粒/玻碳电极（Ag-mSiO NPs/GCE）的无酶传感器表现出良好的电催化性能、重现性和长期稳定性，为原位测定活细胞释放的HO提供了一种成功方法。监测细菌中活性氧（ROS）产生对抗氧化剂和抗生素的影响也可能很有前景。
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