• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于银掺杂介孔二氧化硅纳米颗粒的无酶电化学传感器用于测定活细胞释放的过氧化氢。

Silver Doped Mesoporous Silica Nanoparticles Based Electrochemical Enzyme-Less Sensor for Determination of HO Released from Live Cells.

作者信息

Yang Danting, Ni Ning, Cao Lu, Song Xin, Alhamoud Yasmin, Yu Guangxia, Zhao Jinshun, Zhou Haibo

机构信息

Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medical School of Ningbo University, Ningbo 315211, China.

School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.

出版信息

Micromachines (Basel). 2019 Apr 21;10(4):268. doi: 10.3390/mi10040268.

DOI:10.3390/mi10040268
PMID:31010092
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6523606/
Abstract

In this study, a silver doped mesoporous silica nanoparticles-based enzyme-less electrochemical sensor for the determination of hydrogen peroxide (HO) released from live cells was constructed for the first time. The presented electrochemical sensor exhibited fast response (2 s) towards the reduction of HO concentration variation at an optimized potential of -0.5 V with high selectivity over biological interferents such as uric acid, ascorbic acid, and glucose. In addition, a wide linear range (4 μM to 10 mM) with a low detection limit (LOD) of 3 μM was obtained. Furthermore, the Ag-mSiO nanoparticles/glass carbon electrode (Ag-mSiO NPs/GCE) based enzyme-less sensor showed good electrocatalytic performance, as well as good reproducibility, and long-term stability, which provided a successful way to in situ determine HO released from live cells. It may also be promising to monitor the effect of reactive oxygen species (ROS) production in bacteria against oxidants and antibiotics.

摘要

在本研究中，首次构建了一种基于银掺杂介孔二氧化硅纳米颗粒的无酶电化学传感器，用于测定活细胞释放的过氧化氢（HO）。所展示的电化学传感器在-0.5 V的优化电位下，对HO浓度变化的还原表现出快速响应（2秒），对尿酸、抗坏血酸和葡萄糖等生物干扰物具有高选择性。此外，获得了宽线性范围（4 μM至10 mM），检测限（LOD）低至3 μM。此外，基于Ag-mSiO纳米颗粒/玻碳电极（Ag-mSiO NPs/GCE）的无酶传感器表现出良好的电催化性能、重现性和长期稳定性，为原位测定活细胞释放的HO提供了一种成功方法。监测细菌中活性氧（ROS）产生对抗氧化剂和抗生素的影响也可能很有前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba12/6523606/7ae7a779be64/micromachines-10-00268-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba12/6523606/21551c0ac8ae/micromachines-10-00268-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba12/6523606/2baa24783fa5/micromachines-10-00268-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba12/6523606/463f75b552ac/micromachines-10-00268-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba12/6523606/2f8e249df83a/micromachines-10-00268-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba12/6523606/3e8b3c87f899/micromachines-10-00268-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba12/6523606/7ae7a779be64/micromachines-10-00268-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba12/6523606/21551c0ac8ae/micromachines-10-00268-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba12/6523606/2baa24783fa5/micromachines-10-00268-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba12/6523606/463f75b552ac/micromachines-10-00268-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba12/6523606/2f8e249df83a/micromachines-10-00268-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba12/6523606/3e8b3c87f899/micromachines-10-00268-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba12/6523606/7ae7a779be64/micromachines-10-00268-g006.jpg

相似文献

1
Silver Doped Mesoporous Silica Nanoparticles Based Electrochemical Enzyme-Less Sensor for Determination of HO Released from Live Cells.基于银掺杂介孔二氧化硅纳米颗粒的无酶电化学传感器用于测定活细胞释放的过氧化氢。
Micromachines (Basel). 2019 Apr 21;10(4):268. doi: 10.3390/mi10040268.
2
Ternary nanohybrid of reduced graphene oxide-nafion@silver nanoparticles for boosting the sensor performance in non-enzymatic amperometric detection of hydrogen peroxide.基于还原氧化石墨烯-纳滤@银纳米粒子的三元纳米杂化材料用于提升非酶电化学生物传感器检测过氧化氢性能。
Biosens Bioelectron. 2017 Jan 15;87:1020-1028. doi: 10.1016/j.bios.2016.09.045. Epub 2016 Sep 14.
3
Hydrogen Peroxide Electrochemical Sensor Based on Ag/Cu Bimetallic Nanoparticles Modified on Polypyrrole.基于聚吡咯修饰的Ag/Cu双金属纳米颗粒的过氧化氢电化学传感器。
Sensors (Basel). 2023 Oct 18;23(20):8536. doi: 10.3390/s23208536.
4
Cost-effective three dimensional Ag/polymer dyes/graphene-carbon spheres hybrids for high performance nonenzymatic sensor and its application in living cell HO detection.具有成本效益的三维 Ag/聚合物染料/石墨烯-碳球杂化材料，用于高性能非酶传感器及其在活细胞 HO 检测中的应用。
Bioelectrochemistry. 2018 Oct;123:103-111. doi: 10.1016/j.bioelechem.2018.05.001. Epub 2018 May 3.
5
One-Step Electrodeposition of Silver Nanostructures on 2D/3D Metal-Organic Framework ZIF-67: Comparison and Application in Electrochemical Detection of Hydrogen Peroxide.银纳米结构在二维/三维金属有机框架ZIF-67上的一步电沉积：比较及其在过氧化氢电化学检测中的应用
ACS Appl Mater Interfaces. 2020 Sep 16;12(37):41960-41968. doi: 10.1021/acsami.0c11269. Epub 2020 Aug 26.
6
A highly sensitive hydrogen peroxide sensor based on (Ag-Au NPs)/poly[o-phenylenediamine] modified glassy carbon electrode.基于（Ag-Au NPs）/聚邻苯二胺修饰玻碳电极的高灵敏度过氧化氢传感器。
Mater Sci Eng C Mater Biol Appl. 2015 Nov 1;56:426-31. doi: 10.1016/j.msec.2015.07.001. Epub 2015 Jul 7.
7
Novel Ag@TiO2 nanocomposite synthesized by electrochemically active biofilm for nonenzymatic hydrogen peroxide sensor.电化学活性生物膜合成的新型 Ag@TiO2 纳米复合材料用于非酶过氧化氢传感器。
Mater Sci Eng C Mater Biol Appl. 2013 Dec 1;33(8):4692-9. doi: 10.1016/j.msec.2013.07.028. Epub 2013 Jul 26.
8
Amperometric hydrogen peroxide and glucose biosensor based on NiFe2/ordered mesoporous carbon nanocomposites.基于NiFe2/有序介孔碳纳米复合材料的安培型过氧化氢和葡萄糖生物传感器。
Analyst. 2015 Jan 21;140(2):644-53. doi: 10.1039/c4an01549e.
9
Highly exposed Pt nanoparticles supported on porous graphene for electrochemical detection of hydrogen peroxide in living cells.高度暴露于多孔石墨烯上的 Pt 纳米粒子，用于在活细胞中电化学检测过氧化氢。
Biosens Bioelectron. 2015 Dec 15;74:71-7. doi: 10.1016/j.bios.2015.06.042. Epub 2015 Jun 20.
10
A novel non-enzyme hydrogen peroxide sensor based on catalytic reduction property of silver nanowires.一种基于银纳米线催化还原性质的新型非酶过氧化氢传感器。
Talanta. 2015 Jul 1;139:56-61. doi: 10.1016/j.talanta.2015.02.037. Epub 2015 Feb 26.

引用本文的文献

1
Polyethyleneimine-assisted formation of Ag-SiO hybrid microspheres for HO sensing and SERS applications.用于HO传感和表面增强拉曼光谱应用的聚乙烯亚胺辅助形成Ag-SiO杂化微球。
RSC Adv. 2023 Oct 4;13(42):29086-29098. doi: 10.1039/d3ra04095j.
2
Electrodeposition of Silver Nanoparticles on Indium-Doped Tin Oxide Using Hydrogel Electrolyte for Hydrogen Peroxide Sensing.使用水凝胶电解质在铟掺杂氧化锡上进行银纳米颗粒的电沉积用于过氧化氢传感。
Nanomaterials (Basel). 2022 Dec 22;13(1):48. doi: 10.3390/nano13010048.
3
Recent Trends in Morphology-Controlled Synthesis and Application of Mesoporous Silica Nanoparticles.

本文引用的文献

1
An enzyme-free biosensor for sensitive detection of using curcumin as signal reporter and click chemistry for signal amplification.一种无酶生物传感器，用于灵敏检测，使用姜黄素作为信号报告分子，并采用点击化学进行信号放大。
Theranostics. 2018 Nov 29;8(22):6263-6273. doi: 10.7150/thno.29025. eCollection 2018.
2
Influence of Hydrogen Peroxide, Lactic Acid, and Surfactants from Vaginal Lactobacilli on the Antibiotic Sensitivity of Opportunistic Bacteria.过氧化氢、乳酸以及阴道乳杆菌表面活性剂对机会性细菌抗生素敏感性的影响。
Probiotics Antimicrob Proteins. 2017 Jun;9(2):131-141. doi: 10.1007/s12602-016-9238-6.
3
An ordered mesoporous Ag superstructure synthesized via a template strategy for surface-enhanced Raman spectroscopy.
介孔二氧化硅纳米粒子形态控制合成与应用的最新趋势
Nanomaterials (Basel). 2020 Oct 25;10(11):2122. doi: 10.3390/nano10112122.
4
Synergistic Antibacterial Activity of Silver-Loaded Graphene Oxide towards and .载银氧化石墨烯对[具体对象1]和[具体对象2]的协同抗菌活性 。 （你原文中towards后面内容缺失，请补充完整以便我给出更准确译文）
Nanomaterials (Basel). 2020 Feb 20;10(2):366. doi: 10.3390/nano10020366.
通过模板策略合成的用于表面增强拉曼光谱的有序介孔银超结构。
Nanoscale. 2015 Aug 7;7(29):12318-24. doi: 10.1039/c5nr03759j. Epub 2015 Jul 6.
4
High sensitive and selective sensing of hydrogen peroxide released from pheochromocytoma cells based on Pt-Au bimetallic nanoparticles electrodeposited on reduced graphene sheets.基于电沉积在还原氧化石墨烯片上的铂-金双金属纳米颗粒对嗜铬细胞瘤细胞释放的过氧化氢进行高灵敏度和高选择性传感。
Sensors (Basel). 2015 Jan 26;15(2):2709-22. doi: 10.3390/s150202709.
5
In situ growth of surfactant-free gold nanoparticles on nitrogen-doped graphene quantum dots for electrochemical detection of hydrogen peroxide in biological environments.在氮掺杂石墨烯量子点上原位生长无表面活性剂的金纳米粒子，用于电化学检测生物环境中的过氧化氢。
Anal Chem. 2015 Feb 3;87(3):1903-10. doi: 10.1021/ac5041555. Epub 2015 Jan 9.
6
Green synthesis and applications of Au-Ag bimetallic nanoparticles.金-银双金属纳米粒子的绿色合成与应用
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Feb 25;137:185-92. doi: 10.1016/j.saa.2014.08.079. Epub 2014 Aug 30.
7
Facile, one-pot synthesis, and antibacterial activity of mesoporous silica nanoparticles decorated with well-dispersed silver nanoparticles.介孔硅纳米粒子负载高度分散的银纳米粒子的简便一锅法合成及其抗菌活性。
ACS Appl Mater Interfaces. 2014 Aug 13;6(15):12038-45. doi: 10.1021/am5026424. Epub 2014 Jul 29.
8
In vivo targeting of hydrogen peroxide by activatable cell-penetrating peptides.可激活的细胞穿透肽对过氧化氢的体内靶向作用。
J Am Chem Soc. 2014 Jan 22;136(3):874-7. doi: 10.1021/ja411547j. Epub 2014 Jan 8.
9
Biosensor based on ultrasmall MoS2 nanoparticles for electrochemical detection of H2O2 released by cells at the nanomolar level.基于超小 MoS2 纳米粒子的生物传感器，用于电化学检测纳米摩尔级细胞释放的 H2O2。
Anal Chem. 2013 Nov 5;85(21):10289-95. doi: 10.1021/ac402114c. Epub 2013 Oct 9.
10
Potentiating antibacterial activity by predictably enhancing endogenous microbial ROS production.通过可预测地增强内源性微生物 ROS 产生来增强抗菌活性。
Nat Biotechnol. 2013 Feb;31(2):160-5. doi: 10.1038/nbt.2458. Epub 2013 Jan 6.