Mierzwa Monika, Cytryniak Adrianna, Krysiński Paweł, Bilewicz Renata
Faculty of Chemistry, University of Warsaw, Pasteura 1, PL 02-093 Warsaw, Poland.
Nanomaterials (Basel). 2019 Apr 19;9(4):636. doi: 10.3390/nano9040636.
The release profiles of methotrexate, an anticancer drug, from the monoolein liquid crystalline cubic phases were studied. The cubic phases were used either in the form of a lipidic film deposited onto a glassy carbon electrode surface or in the dispersed form of magnetocubosomes, which are considered a prospective hybrid drug delivery system. Commonly, cubosomes or liposomes are employed, but not in the case of toxic methotrexate, known to block the receptors responsible for folate transport into the cells. The release profiles of the drug from the lipidic films were monitored electrochemically and described using the Higuchi model. They were also modified via changes in temperature; the release was faster, although it deviated from the model when the temperature was increased. Cubic phase nanoparticles (magnetocubosomes) containing hydrophobic magnetic nanoparticles placed in an alternating magnetic field of low frequency and amplitude, stimulated drug release from the suspension, which was monitored spectroscopically. These new biocompatible hybrid nanomaterials in the dispersed form allow to control the release of the drug at the appropriate sites, can be easily separated or relocated under external magnetic field and await further investigations of their in vitro cytotoxicity and in vivo biodistribution.
研究了抗癌药物甲氨蝶呤从单油酸液晶立方相中的释放曲线。立方相既可以以沉积在玻碳电极表面的脂质膜形式使用，也可以以磁立方体制剂的分散形式使用，磁立方体制剂被认为是一种有前景的混合药物递送系统。通常使用立方体制剂或脂质体，但对于已知会阻断负责叶酸转运进入细胞的受体的有毒甲氨蝶呤则不然。通过电化学监测药物从脂质膜中的释放曲线，并使用Higuchi模型进行描述。它们也通过温度变化进行了改性；释放速度更快，尽管温度升高时偏离了模型。含有疏水性磁性纳米颗粒的立方相纳米颗粒（磁立方体制剂）置于低频和低振幅交变磁场中，刺激了悬浮液中药物的释放，通过光谱进行监测。这些分散形式的新型生物相容性混合纳米材料能够在适当部位控制药物释放，在外部磁场作用下可以很容易地分离或重新定位，有待进一步研究其体外细胞毒性和体内生物分布。
