Institute for Biologically Inspired Materials, Department of Nanobiotechnology, University of Natural Resources and Life Sciences, Muthgasse 11, 1190, Vienna, Austria.
Sci Rep. 2017 Aug 7;7(1):7474. doi: 10.1038/s41598-017-06980-9.
Stealth (PEGylated) liposomes have taken a central role in drug formulation and delivery combining efficient transport with low nonspecific interactions. Controlling rapid release at a certain location and time remains a challenge dependent on environmental factors. We demonstrate a highly efficient and scalable way to produce liposomes of any lipid composition containing homogeneously dispersed monodisperse superparamagnetic iron oxide nanoparticles in the membrane interior. We investigate the effect of lipid composition, particle concentration and magnetic field actuation on colloidal stability, magneto-thermally actuated release and passive release rates. We show that the rate and amount of encapsulated hydrophilic compound released by actuation using alternating magnetic fields can be precisely controlled from stealth liposomes with high membrane melting temperature. Extraordinarily low passive release and temperature sensitivity at body temperature makes this a promising encapsulation and external-trigger-on-demand release system. The introduced feature can be used as an add-on to existing stealth liposome drug delivery technology.
隐形（聚乙二醇化）脂质体在药物制剂和递送上发挥了核心作用，将高效运输与低非特异性相互作用结合在一起。控制在特定位置和时间的快速释放仍然是一个依赖于环境因素的挑战。我们展示了一种高效且可扩展的方法，可用于生产任何脂质组成的脂质体，其中在膜内部均匀分散有单分散超顺磁性氧化铁纳米颗粒。我们研究了脂质组成、颗粒浓度和磁场驱动对胶体稳定性、磁热驱动释放和被动释放速率的影响。我们表明，使用交变磁场进行驱动时，封装亲水性化合物的释放速率和释放量可以从具有高膜熔化温度的隐形脂质体中进行精确控制。极低的被动释放和在体温下的温度敏感性使得这成为一种有前途的封装和外部触发按需释放系统。所引入的特征可用作现有隐形脂质体药物输送技术的附加功能。
