Peng Wei, Lei Mengtong, Zhang Jun, Zhang Yong
1College of Veterinary Medicine,Northwest A&F University,Yangling 712100,Shaanxi,China.
2Institute of Veterinary Medicine,Qinghai Academy of Animal Science and Veterinary Medicine,Xining,Qinghai,China.
Zygote. 2019 Jun;27(3):118-125. doi: 10.1017/S0967199418000412. Epub 2019 Apr 23.
SummaryMelatonin plays a critical role in several types of cells as an antioxidant to protect intracellular molecules from oxidative stress. The anti-oxidation effect of melatonin in yak embryos is largely unknown. We report that melatonin can protect the development of yak preimplantation embryos against oxidative stress induced by hydrogen peroxide (H2O2). Therefore, the quality of blastocysts developed from zygotes exposed to H2O2 was promoted. In addition, we observed that melatonin reduced H2O2-induced intracellular reactive oxygen species (ROS) levels and prevented mitochondrial dysfunction in zygotes. These phenomena revealed the effective antioxidant activity of melatonin to prevent oxidative stress in yak embryos. To determine the underlying mechanism, we further demonstrated that melatonin protected preimplantation embryos from oxidative damage by preserving antioxidative enzymes. Collectively, these results confirmed the anti-oxidation effect of melatonin in yak embryos that significantly improved the quantity and quality of blastocysts in the in vitro production of embryos in yaks.
摘要
褪黑素作为一种抗氧化剂，在多种细胞类型中发挥着关键作用，可保护细胞内分子免受氧化应激的影响。褪黑素对牦牛胚胎的抗氧化作用在很大程度上尚不清楚。我们报道，褪黑素可以保护牦牛植入前胚胎的发育免受过氧化氢（H2O2）诱导的氧化应激。因此，由暴露于H2O2的受精卵发育而来的囊胚质量得到了提高。此外，我们观察到褪黑素降低了H2O2诱导的受精卵内活性氧（ROS）水平，并预防了线粒体功能障碍。这些现象揭示了褪黑素在预防牦牛胚胎氧化应激方面的有效抗氧化活性。为了确定潜在机制，我们进一步证明，褪黑素通过保留抗氧化酶来保护植入前胚胎免受氧化损伤。总的来说，这些结果证实了褪黑素在牦牛胚胎中的抗氧化作用，显著提高了牦牛胚胎体外生产中囊胚的数量和质量。
