Department of Animal Science, Chungbuk National University, Cheongju, South Korea.
College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Sci Rep. 2017 Dec 5;7(1):16992. doi: 10.1038/s41598-017-17287-0.
C-Phycocyanin (CP) is a biliprotein enriched in blue-green algae that is known to possess antioxidant, anti-apoptosis, anti-inflammatory, and radical-scavenging properties in somatic cells. However, the protective effect of CP on porcine embryo developmental competence in vitro remains unclear. In the present study, we investigated the effect of CP on the development of early porcine embryos as well as its underlying mechanisms. Different concentrations of CP (2, 5, 8, 10 μg/mL) were added to porcine zygote medium 5 during in vitro culture. The results showed that 5 μg/mL CP significantly increased blastocyst formation and hatching rate. Blastocyst formation and quality were significantly increased in the 50 μM HO treatment group following 5 μg/mL CP addition. CP prevented the HO-induced compromise of mitochondrial membrane potential, release of cytochrome c from the mitochondria, and reactive oxygen species generation. Furthermore, apoptosis, DNA damage level, and autophagy in the blastocysts were attenuated by supplementation of CP in the HO-induced oxidative injury group compared to in controls. These results suggest that CP has beneficial effects on the development of porcine parthenotes by attenuating mitochondrial dysfunction and oxidative stress.
藻蓝蛋白（CP）是一种富含蓝绿藻的胆色素蛋白，已知在体细胞中具有抗氧化、抗凋亡、抗炎和自由基清除特性。然而，CP 对体外猪胚胎发育能力的保护作用尚不清楚。在本研究中，我们研究了 CP 对早期猪胚胎发育的影响及其潜在机制。在体外培养过程中，将不同浓度的 CP（2、5、8、10μg/mL）添加到猪受精卵培养基 5 中。结果表明，5μg/mL CP 可显著提高囊胚形成率和孵化率。添加 5μg/mL CP 后，50μM HO 处理组的囊胚形成和质量显著增加。CP 可防止 HO 诱导的线粒体膜电位崩溃、细胞色素 c 从线粒体释放以及活性氧生成。此外，与对照组相比，HO 诱导的氧化损伤组中 CP 的补充可减轻凋亡、DNA 损伤水平和囊胚中的自噬。这些结果表明，CP 通过减轻线粒体功能障碍和氧化应激对猪孤雌胚胎的发育具有有益作用。
