Huang Minjie, Chen Lixing, Shen Yifei, Chen Jiucheng, Guo Xiaoling, Xu Ningying
Department of Animal Genetics and Breeding, College of Animal Science, Zhejiang University, Hangzhou 310058, China.
Asian-Australas J Anim Sci. 2019 Mar 7;32(10):1483-1490. doi: 10.5713/ajas.18.0807. Print 2019 Oct.
To explore the molecular mechanisms of fat metabolism and deposition in pigs, an experiment was conducted to identify hepatic mRNAs and miRNAs expression and determine the potential interaction of them in two phenotypically extreme pig breeds.
mRNA and miRNA profiling of liver from 70-day Jinhua (JH) and Landrace (LD) pigs were performed using RNA sequencing. Blood samples were taken to detect results of serum biochemistry. Bioinformatics analysis were applied to construct differentially expressed miRNA-mRNA network.
Serum total triiodothyronine (TT3) and total thyroxine (TT4) were significantly lower in Jinhua pigs, but the content of serum total cholesterol (TCH) and low-density lipoprotein cholesterol (LDLC) were strikingly higher. A total of 467 differentially expressed genes (DEGs) and 35 differentially expressed miRNAs (DE miRNAs) were identified between JH and LD groups. Gene ontology analysis suggested that DEGs were involved in oxidation-reduction, lipid biosynthetic and lipid metabolism process. Interaction network of DEGs and DE miRNAs were constructed, according to target prediction results.
We generated transcriptome and miRNAome profiles of liver from Jinhua and Landrace pig breeds which represent distinguishing phenotypes of growth and metabolism. The potential miRNA-mRNA interaction networks may provide a comprehensive understanding in the mechanism of lipid metabolism. These results serve as a basis for further investigation on biological functions of miRNAs in the porcine liver.
为探究猪脂肪代谢和沉积的分子机制，开展了一项实验，以鉴定两个表型差异极大的猪品种肝脏中的mRNA和miRNA表达情况，并确定它们之间的潜在相互作用。
采用RNA测序技术对70日龄的金华猪（JH）和长白猪（LD）肝脏进行mRNA和miRNA谱分析。采集血样检测血清生化结果。应用生物信息学分析构建差异表达的miRNA-mRNA网络。
金华猪血清总三碘甲状腺原氨酸（TT3）和总甲状腺素（TT4）显著较低，但血清总胆固醇（TCH）和低密度脂蛋白胆固醇（LDLC）含量显著较高。在JH和LD组之间共鉴定出467个差异表达基因（DEGs）和35个差异表达miRNA（DE miRNAs）。基因本体分析表明，DEGs参与氧化还原、脂质生物合成和脂质代谢过程。根据靶标预测结果构建了DEGs和DE miRNAs的相互作用网络。
我们生成了金华猪和长白猪肝脏的转录组和miRNA组图谱，这两个品种代表了生长和代谢方面不同的表型。潜在的miRNA-mRNA相互作用网络可能为脂质代谢机制提供全面的理解。这些结果为进一步研究miRNA在猪肝中的生物学功能奠定了基础。
