罗氏Integra 400 plus与雅培Architect ci8200在丙氨酸氨基转移酶检测中的比较。

Comparison between Roche Integra 400 plus and Abbott Architect ci8200 in Alanine aminotransferase assay.

作者信息

Lamrabat Somiya, Lyagoubi Amina, Bouayadi Ouardia, Elmalki Jalila, Elkouche Khadija, Bensalah Mohammed, Choukri Mohammed

机构信息

Central Laboratory of Mohammed VI University Hospital of Oujda City, Morocco.

Faculty of Medicine and Pharmacy, First Mohammed University, Oujda City, Morocco.

出版信息

Pract Lab Med. 2019 Mar 22;15:e00121. doi: 10.1016/j.plabm.2019.e00121. eCollection 2019 May.

DOI:10.1016/j.plabm.2019.e00121

PMID:31011608

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6462767/

Abstract

BACKGROUND

The aim of this work is to present the results of a comparative study between the ALT assay on Integra 400 plus Roche Diagnostic versus Architect ci8200 of Abbott Diagnostic.

METHODS

A total of 200 patients hospitalized in the various departments of the university hospital Mohammed VI of Oujda were prospectively tested on two systems: Abbott Architect ci8200 and Roche Integra 400 plus. Both analyzers use the spectrophotometric technique by coupling the transamination reaction to the oxidation-reduction reaction at NAD. The agreement of the results between the different techniques was evaluated using the Bland-Altman difference diagram and the Passing-Bablok and Deming regression line.

RESULTS

There was a high concordance between the two assays: the equation of the Passing-Bablok line is YArchitect = -0,5625 + 0,9917 XIntegra with a correlation coefficient r = 0.999. The Bland-Altman diagram shows that the mean bias between the two methods is of the order of 1.1 IU/L and the difference between the ALT measurements by Architect and Integra is in the range of -1.4 to 3.6.

CONCLUSION

Our study shows a high correlation of the ALT assay results between the architect ci8200 and Integra 400 plus.

摘要

背景

本研究旨在呈现罗氏诊断公司的 Integra 400 plus 与雅培诊断公司的 Architect ci8200 上谷丙转氨酶（ALT）检测结果的比较研究成果。

方法

对乌季达穆罕默德六世大学医院各科室收治的 200 例患者，前瞻性地在两种系统上进行检测：雅培 Architect ci8200 和罗氏 Integra 400 plus。两种分析仪均采用分光光度技术，通过将转氨反应与 NAD 处的氧化还原反应偶联。使用 Bland-Altman 差异图以及 Passing-Bablok 和 Deming 回归线评估不同技术间结果的一致性。

结果

两种检测方法之间具有高度一致性：Passing-Bablok 线的方程为 YArchitect = -0.5625 + 0.9917 XIntegra，相关系数 r = 0.999。Bland-Altman 图显示，两种方法之间的平均偏差约为 1.1 IU/L，Architect 和 Integra 检测 ALT 的差异范围在 -1.4 至 3.6 之间。