Department of Surgery, Division of Urology, Human Reproduction Section, Sao Paulo Federal University, Rua Embau, 231, CEP: 04039-060, Sao Paulo, SP, Brazil.
Fertility Medical Group, Av. Brigadeiro Luiz Antônio, 4545, CEP: 04511-010, Sao Paulo, SP, Brazil.
J Assist Reprod Genet. 2019 May;36(5):1003-1011. doi: 10.1007/s10815-019-01428-7. Epub 2019 Apr 23.
The present study aimed to provide a non-invasive approach to studying mechanisms responsible for oocyte development.
To this end, follicular fluid (FF) from 62 patients undergoing in vitro fertilization (IVF) cycles was split into two groups depending on the pregnancy outcome: pregnant (n = 28) and non-pregnant (n = 34) groups. Data were acquired by the MALDI-TOF mass spectrometry. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were applied to the data set. A ROC curve, to predict success rate, was constructed, and the lipids were attributed.
Six ions were differentially represented in FF of pregnant and non-pregnant patients, with an area under the curve of 0.962. Phosphatidic acid, phosphatidylglycerol, and triacylglycerol were hyper-represented in the pregnant group, while glucosylceramide was hyper-represented in the non-pregnant group. Enriched functions related to these lipids are steroidogenesis, cellular response, signal transduction, cell cycle, and activation of protein kinase C for the pregnant group and apoptosis inhibition for the non-pregnant group.
Human FF fingerprinting can both improve the understanding concerning mechanisms responsible for oocyte development and its effect on embryo implantation potential and assist in the management of IVF cycles.
本研究旨在提供一种非侵入性的方法来研究卵母细胞发育的机制。
为此，将 62 名接受体外受精 (IVF) 周期的患者的卵泡液 (FF) 分为两组，根据妊娠结局：妊娠组 (n=28) 和非妊娠组 (n=34)。通过 MALDI-TOF 质谱获取数据。应用主成分分析 (PCA) 和正交偏最小二乘判别分析 (OPLS-DA) 对数据集进行分析。构建了预测成功率的 ROC 曲线，并对脂质进行了归属。
妊娠和非妊娠患者的 FF 中有 6 个离子存在差异表达，曲线下面积为 0.962。妊娠组中磷酸脂酰甘油、磷脂酰甘油和三酰甘油表达上调，而非妊娠组中葡萄糖脑苷脂表达上调。与这些脂质相关的富集功能包括类固醇生成、细胞反应、信号转导、细胞周期和蛋白激酶 C 的激活，用于妊娠组，以及凋亡抑制，用于非妊娠组。
人 FF 指纹图谱不仅可以提高对卵母细胞发育及其对胚胎着床潜能影响的机制的理解，还可以帮助管理 IVF 周期。
