鲤水肿病毒（CEV）的潜在媒介物种。
Potential vector species of carp edema virus (CEV).
作者信息
Matras Marek, Stachnik Magdalena, Borzym Ewa, Maj-Paluch Joanna, Reichert Michal
机构信息
Department of Fish Diseases, National Veterinary Research Institute, Pulawy, Poland.
出版信息
J Fish Dis. 2019 Jul;42(7):959-964. doi: 10.1111/jfd.13000. Epub 2019 Apr 23.
During a PCR-based CEV survey in Poland in 2015-2017, the virus was detected in many farms both in clinical and asymptomatic cases and in common as well as in koi carp (Cyprinus carpio). In order to evaluate the potential carrier role of fish species that share the same habitats with carp, an experimental trial was performed. Investigations carried out on specimens of bleak (Alburnus alburnus), crucian carp (Carassius carassius), European perch (Perca fluviatilis), Prussian carp (Carassius gibelio), roach (Rutilus rutilus) and tench (Tinca tinca) cohabited with CEV-infected carp yielded positive results. These species of fish were experimentally cohabited with CEV-infected common carp at a temperature of 16°C ± 1. Material from the brain, gills, spleen, kidneys, intestine and skin was investigated for the presence of CEV DNA. Similar investigations were performed with uninfected fish designated controls. Samples were tested for CEV by qPCR.
在2015年至2017年于波兰开展的一项基于聚合酶链反应（PCR）的鲤鱼水肿病毒（CEV）调查中，该病毒在许多养殖场的临床病例和无症状病例中均被检测到，且在普通鲤鱼以及锦鲤（Cyprinus carpio）中也有发现。为了评估与鲤鱼共享相同栖息地的鱼类物种作为潜在病毒携带者的作用，开展了一项试验。对与感染CEV的鲤鱼共同生活的银鲴（Alburnus alburnus）、鲫鱼（Carassius carassius）、欧洲鲈鱼（Perca fluviatilis）、镜鲤（Carassius gibelio）、拟赤梢鱼（Rutilus rutilus）和丁鱥（Tinca tinca）样本进行的调查得出了阳性结果。这些鱼类物种在16°C ± 1的温度下与感染CEV的鲤鱼进行了试验性共同养殖。对取自大脑、鳃、脾脏、肾脏、肠道和皮肤的样本进行了CEV DNA检测。对指定为对照的未感染鱼类也进行了类似调查。通过定量聚合酶链反应（qPCR）对样本进行CEV检测。
Zotero 插件