Orfali Raha, Perveen Shagufta, Siddiqui Nasir Ali, Alam Perwez, Alhowiriny Tawfeq Abdullah, Al-Taweel Areej Mohammad, Al-Yahya Sami, Ameen Fuad, Majrashi Najwa, Alluhayb Khulud, Alghanem Bandar, Shaibah Hayat, Khan Shabana Iqrar
Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
National Center for Biotechnology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh 11461, Saudi Arabia.
J Anal Methods Chem. 2019 Mar 19;2019:7435909. doi: 10.1155/2019/7435909. eCollection 2019.
is an important genus of the Loranthaceae family, and it is a semiparasitic plant grown in Saudi Arabia, traditionally used as a cure for diabetes and cancer in human and for increasing lactation in cattle. A flavonoid quercetin (), (-)-catechin (), and a flavane gallate 2S,3R-3,3',4',5,7-pentahydroxyflavane-5-O-gallate () were isolated from the methanol extract of the aerial parts of (PCME). The PCME and the isolated compounds were subjected to pharmacological assays to estimate peroxisome proliferator-activated receptors PPAR and PPAR agonistic, anti-inflammatory, cytotoxic, and antimicrobial activities. Results proved for the first time the dual PPAR activation effect of the PCME and catechin (), in addition to the promising anti-inflammatory activity of the flavonoid quercetin (). Interestingly, both PCME and isolated compounds showed potent antioxidant activities while no antimicrobial effect against certain microbial strains had been reported from the extract and the isolated compounds. Based on the pharmacological importance of these compounds, an HPTLC validated method was developed for the simultaneous estimation of these compounds in PCME. It was found to furnish a compact and sharp band of compounds , , and at = 0.34, 0.47, and 0.65, respectively, using dichloromethane, methanol, and formic acid (90 : 9.5 : 0.5, (v/v/v)) as the mobile phase. Compounds , , and were found to be 11.06, 10.9, 6.96 g/mg, respectively, in PCME. The proposed HPTLC method offers a sensitive, precise, and specific analytical tool for the quantification of quercetin, catechin, and flavane gallates in .
是桑寄生科的一个重要属，是一种生长在沙特阿拉伯的半寄生植物，传统上用于治疗人类的糖尿病和癌症以及增加牛的泌乳量。从（PCME）地上部分的甲醇提取物中分离出一种黄酮类化合物槲皮素（）、（-）-儿茶素（）和一种黄烷没食子酸酯2S,3R-3,3',4',5,7-五羟基黄烷-5-O-没食子酸酯（）。对PCME和分离出的化合物进行药理分析，以评估过氧化物酶体增殖物激活受体PPAR和PPAR的激动、抗炎、细胞毒性和抗菌活性。结果首次证明了PCME和儿茶素（）的双重PPAR激活作用，此外，黄酮类化合物槲皮素（）具有良好的抗炎活性。有趣的是，PCME和分离出的化合物均显示出强大的抗氧化活性，而提取物和分离出的化合物对某些微生物菌株均未报告有抗菌作用。基于这些化合物的药理重要性，开发了一种经过HPTLC验证的方法，用于同时测定PCME中的这些化合物。结果发现，以二氯甲烷、甲醇和甲酸（90 : 9.5 : 0.5，（v/v/v））为流动相时，化合物、和分别在 = 0.34、0.47和0.65处呈现出紧密且清晰的斑点。在PCME中，化合物、和的含量分别为11.06、10.9、6.96 g/mg。所提出的HPTLC方法为定量测定中的槲皮素、儿茶素和黄烷没食子酸酯提供了一种灵敏、精确且特异的分析工具。
