Jahn B, Burmester G R, Schmid H, Weseloh G, Rohwer P, Kalden J R
Arthritis Rheum. 1987 Jan;30(1):64-74. doi: 10.1002/art.1780300109.
Ia antigens (class II HLA molecules) have been detected on cells eluted from affected human cartilage in certain disease states, but not on normal cartilage cells. Because the presence of Ia antigens on chondrocytes may play an important role in rheumatic diseases, we investigated the induction of these molecules by gamma-interferon (gamma-IFN), a potent Ia-inducing lymphokine. Human articular chondrocytes were incubated with recombinant gamma-IFN, and the expression of Ia antigens was studied by cell sorter analysis, using a panel of reagents that detect monomorphic and polymorphic specificities of the DR and DQ Ia antigen families. While the induction of DR antigens, including polymorphic DR specificities, was readily obtained with gamma-IFN (50-95% positive cells), DQ antigens were negative or were displayed only on a lower percentage of chondrocytes (5-60%). In addition, incubation with gamma-IFN led to an increased expression of HLA class I antigens. The expression of various other surface markers either remained unchanged (as in 4F2 and BA-2) or showed tendencies toward decreased percentages (as in 83c2) or increased percentages (as in M phi R-17). No apparent change in cell morphology or growth pattern was observed.
在某些疾病状态下，已在从受累人类软骨洗脱的细胞上检测到Ia抗原（II类HLA分子），但正常软骨细胞上未检测到。由于软骨细胞上Ia抗原的存在可能在风湿性疾病中起重要作用，我们研究了γ干扰素（γ-IFN）对这些分子的诱导作用，γ-IFN是一种有效的Ia诱导淋巴细胞因子。将人关节软骨细胞与重组γ-IFN孵育，使用一组检测DR和DQ Ia抗原家族单态性和多态性特异性的试剂，通过细胞分选分析研究Ia抗原的表达。虽然用γ-IFN很容易诱导出DR抗原，包括多态性DR特异性（50 - 95%阳性细胞），但DQ抗原呈阴性或仅在较低百分比的软骨细胞上显示（5 - 60%）。此外，与γ-IFN孵育导致HLA I类抗原表达增加。各种其他表面标志物的表达要么保持不变（如4F2和BA - 2），要么显示出百分比下降的趋势（如83c2）或增加的趋势（如M phi R - 17）。未观察到细胞形态或生长模式有明显变化。
