Sprotte Sabrina, Brinks Erik, Neve Horst, Franz Charles M A P
Department of Microbiology and Biotechnology, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Hermann-Weigmann-Str. 1, 24103, Kiel, Germany.
Heliyon. 2024 Apr 5;10(8):e28813. doi: 10.1016/j.heliyon.2024.e28813. eCollection 2024 Apr 30.
PMBT24, the first reported virulent bacteriophage infecting the anaerobic human gut bacterium strain MBT-21, was isolated from a municipal sewage sample and its genome was sequenced and analysed. Transmission electron microscopy revealed a phage with an icosahedral head and a long, non-contractile tail. The circularly permutated, 99,962-bp dsDNA genome of the type phage has a mol% G + C content of 32.1 and comprises 173 putative ORFs. Using amino acid sequence-based phylogeny, phage PMBT24 showed similarity to other, hitherto non-published phage genomes in the databases. Our data suggested phage PMBT24 to present the type phage of a novel genus (proposed name Kielvirus) and novel family of phages (proposed name Kielviridae).
PMBT24是首个被报道的可感染人类厌氧肠道细菌MBT - 21菌株的烈性噬菌体，它从城市污水样本中分离出来，其基因组已被测序和分析。透射电子显微镜显示该噬菌体具有二十面体头部和一条长的、非收缩性的尾部。该噬菌体类型的环状排列的99,962碱基对双链DNA基因组的G + C摩尔百分比含量为32.1，包含173个推定的开放阅读框。基于氨基酸序列的系统发育分析表明，噬菌体PMBT24与数据库中其他尚未发表的噬菌体基因组具有相似性。我们的数据表明噬菌体PMBT24是一个新属（提议名称为Kielvirus）和噬菌体新科（提议名称为Kielviridae）的模式噬菌体。
