Beijing Key Laboratory of Plants Resource Research and Development, School of Sciences, Beijing Technology and Business University, Beijing, China.
Aerospace Center Hospital, Cardiovascular Department, Beijing, China.
PLoS One. 2019 Apr 25;14(4):e0216082. doi: 10.1371/journal.pone.0216082. eCollection 2019.
The elicitor Hrip1 isolated from necrotrophic fungus Alternaria tenuissima, could induce systemic acquired resistance in tobacco to enhance resistance to tobacco mosaic virus. In the present study, we found that the transgenic lines of Hrip1-overexpression in wild type (WT) Arabidopsis thaliana were more resistant to Spodoptera exigua and were early bolting and flowering than the WT. A profiling of transcription assay using digital gene expression profiling was used for transgenic and WT Arabidopsis thaliana. Differentially expressed genes including 40 upregulated and three downregulated genes were identified. In transgenic lines of Hrip1-overexpression, three genes related to jasmonate (JA) biosynthesis were significantly upregulated, and the JA level was found to be higher than WT. Two GDSL family members (GLIP1 and GLIP4) and pathogen-related gene, which participated in pathogen defense action, were upregulated in the transgenic line of Hrip1-overexpression. Thus, Hrip1 is involved in affecting the flower bolting time and regulating endogenous JA biosynthesis and regulatory network to enhance resistance to insect.
从坏死真菌细交链孢菌中分离出的激发子 Hrip1 能够诱导烟草中的系统获得性抗性，从而增强对烟草花叶病毒的抗性。在本研究中，我们发现 Hrip1 过表达的转基因拟南芥品系比野生型（WT）拟南芥对甜菜夜蛾更具抗性，并且早开花和开花。使用数字基因表达谱进行了转基因和 WT 拟南芥的转录谱分析。鉴定出包括 40 个上调和 3 个下调基因在内的差异表达基因。在 Hrip1 过表达的转基因系中，与茉莉酸（JA）生物合成相关的三个基因显著上调，并且发现 JA 水平高于 WT。两个 GDSL 家族成员（GLIP1 和 GLIP4）和参与抗病防御作用的病程相关基因在 Hrip1 过表达的转基因系中上调。因此，Hrip1 参与影响开花时间，调节内源 JA 生物合成和调控网络，以增强对昆虫的抗性。
