Department of Chemistry, Faculty of Engineering, Istanbul University-Cerrahpasa, Istanbul, Turkey.
Department of Molecular Biology and Genetics, Uskudar University, Istanbul, Turkey.
J Biochem Mol Toxicol. 2019 Aug;33(8):e22347. doi: 10.1002/jbt.22347. Epub 2019 Apr 25.
Brain damage is a major complication of fulminant hepatic failure. d-Galactosamine (d-GalN)-induced liver toxicity causes damage to brain. The effects of vitamins and selenium mixture against d-GalN stimulated brain injury were investigated in this study. Sprague-Dawley female rats aged 2.0-2.5 months were used for the study. The rats were divided into four categories. A 0.9% NaCl solution was intraperitoneally given to the experimental rats in the first group. Using gavage technique, the second group of animals were subjected to a formulation consisting of 100 mg·kg ·day vitamin C, 15 mg·kg ·day of β-carotene, 100 mg·kg ·day of α-tocopherol in addition to 0.2 mg·kg ·day of sodium selenate for 3 days. The third group was given a single dose of d-GalN hydrochloride at the concentration of 500 mg·kg through a saline injection. The final group was given similar concentrations of both the antioxidant combination and d-GalN. Tissue samples were collected under ether anesthesia. The rats treated with d-GalN showed brain damage; increased myeloperoxidase, catalase, glutathione peroxidase, glutathione-S-transferase, lactate dehydrogenase, and superoxide dismutase activities; and decreased glutathione levels. Treatment with vitamins and selenium combination resulted in alleviation of these alterations in the rats. These findings suggest that administration of the vitamins and selenium combination suppresses oxidative stress and protects brain cells from injury induced by d-GalN.
脑损伤是暴发性肝衰竭的主要并发症。半乳糖胺（d-GalN）诱导的肝毒性可导致脑损伤。本研究旨在探讨维生素和硒混合物对 d-GalN 刺激脑损伤的作用。将 2.0-2.5 月龄的 Sprague-Dawley 雌性大鼠用于本研究。将大鼠分为四组。第一组实验大鼠腹腔内给予 0.9%氯化钠溶液。第二组动物通过灌胃技术给予包含 100mg·kg·day 维生素 C、15mg·kg·day β-胡萝卜素、100mg·kg·day α-生育酚以及 0.2mg·kg·day 亚硒酸钠的配方 3 天。第三组大鼠通过生理盐水注射给予 500mg·kg 浓度的盐酸半乳糖胺单次剂量。最后一组大鼠给予相同浓度的抗氧化剂混合物和 d-GalN。在乙醚麻醉下收集组织样本。用 d-GalN 处理的大鼠表现出脑损伤；髓过氧化物酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽-S-转移酶、乳酸脱氢酶和超氧化物歧化酶活性增加；谷胱甘肽水平降低。给予维生素和硒混合物治疗可减轻这些改变。这些发现表明，给予维生素和硒混合物可抑制氧化应激，保护脑细胞免受 d-GalN 诱导的损伤。
