槐米提取物对链脲佐菌素诱导的糖尿病大鼠的降糖和肾保护作用。
Anti-diabetic and renoprotective effects of Cassiae Semen extract in the streptozotocin-induced diabetic rats.
机构信息
Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, College of Pharmacy, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, 430030, China.
Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, College of Pharmacy, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, 430030, China.
出版信息
J Ethnopharmacol. 2019 Jul 15;239:111904. doi: 10.1016/j.jep.2019.111904. Epub 2019 Apr 22.
ETHNOPHARMACOLOGICAL RELEVANCE
Cassiae Semen, the dried seed of Cassia obtusifolia L. (Leguminosae), is a traditional Chinese medicine. It has long been used as the treatment of diabetic hyperlipidemia and diabetic constipation in Traditional Chinese Medicine formulae.
AIM OF THE STUDY
The present study was designed to investigate the anti-diabetic and renoprotective effects of Cassiae Semen extract (CSE) in streptozotocin (STZ)-induced diabetic rats.
MATERIALS AND METHODS
Quality control of CSE was performed using HPLC. CSE were orally administered at 27, 54 and 81 mg/kg dose to high-sucrose-high-fat (HSHF) diet and STZ-induced diabetic rats for 60 days. Body weight, glucose metabolism and lipid metabolism profiles were measured to assess the anti-diabetic effect of CSE. Oxidative stress markers and inflammatory factors were determined using commercial kits. Renal function related parameters were also measured. Histopathological examination of kidney was conducted for the validation of pathological changes in the diabetic rats. Immunohistochemical examination of kidney was measured to investigate the expression of RAGE in renal tissues.
RESULTS
Five compounds, including two anthraquinones and three naphtopyrones were simultaneously determined in CSE. Compared with diabetic control, groups treated with CSE exhibited an anti-diabetic effect, including a significant amelioration in body weight, glycemic control, oral glucose tolerance and lipid metabolism (P < 0.01). Moreover, oxidative stress and inflammatory responses decreased after oral administration of CSE (P < 0.01). CSE also showed protective effects on renal functions, decreasing the ratio of kidney/body weight, 24 h urine volume, 24 h urine protein, serum creatinine (Scr) and blood urea nitrogen (BUN) (P < 0.01). Additionally, renal protective effect was also observed in histopathological examination. Immunohistochemical analysis showed that CSE downregulated the expression of RAGE.
CONCLUSIONS
It turned out that CSE had both anti-diabetic and renoprotective effects in diabetic rats. CSE can be a potential agent in the treatment of type 2 diabetes mellitus (T2DM) and its complications.
民族药理学相关性
决明子，即钝叶决明的干燥种子（豆科），是一种传统中药。它一直被用于中药方剂中治疗糖尿病高脂血症和糖尿病性便秘。
研究目的
本研究旨在探讨决明子提取物（CSE）对链脲佐菌素（STZ）诱导的糖尿病大鼠的抗糖尿病和肾脏保护作用。
材料和方法
采用高效液相色谱法对 CSE 进行质量控制。CSE 以 27、54 和 81mg/kg 剂量口服给予高蔗糖高脂肪（HSHF）饮食和 STZ 诱导的糖尿病大鼠 60 天。测量体重、葡萄糖代谢和脂质代谢谱，以评估 CSE 的抗糖尿病作用。使用商业试剂盒测定氧化应激标志物和炎症因子。还测量了肾功能相关参数。对肾脏进行组织病理学检查，以验证糖尿病大鼠的病理变化。免疫组织化学检查肾脏，以研究 RAGE 在肾脏组织中的表达。
结果
在 CSE 中同时测定了五种化合物，包括两种蒽醌和三种萘醌。与糖尿病对照组相比，CSE 治疗组表现出抗糖尿病作用，包括体重、血糖控制、口服葡萄糖耐量和脂质代谢显著改善（P<0.01）。此外，CSE 口服后氧化应激和炎症反应降低（P<0.01）。CSE 还对肾功能具有保护作用，降低肾/体重比、24 小时尿量、24 小时尿蛋白、血清肌酐（Scr）和血尿素氮（BUN）（P<0.01）。此外，在组织病理学检查中也观察到肾脏保护作用。免疫组织化学分析显示，CSE 下调了 RAGE 的表达。
结论
结果表明，CSE 对糖尿病大鼠具有抗糖尿病和肾脏保护作用。CSE 可以成为治疗 2 型糖尿病（T2DM）及其并发症的潜在药物。
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